«ALVETSENSE» Real-Time PCR kit
«ALVETSENSE» Real-Time PCR kit for the SARS-CoV-2 qualitative detection by RT-PCR in real time (cat. No. AL-SARSV-96) is intended for the 2019-nCoV coronavirus RNA detection by a one-step polymerase chain reaction with fluorescence detection in real time (hereinafter RT-PCR-RT) in nucleic acid preparations isolated from smear samples from the external and internal surfaces of food packaging, containers and the product itself, hereinafter referred to as the kit. The kit detects the following genes: 3 loci of the orf1ab gene of the 2019-nCoV coronavirus and the S, E and N genes of the 2019-nCoV coronavirus.
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The kit is used with the following recording amplifier models: CFX96, iQ5 (manufactured by Bio-Rad, Singapore / USA); DT-322, DTprime, DTlight (manufactured by NPO DNA-technology LLC (ООО «НПО ДНК-технология»), Russia); Rotor-Gene Q, Rotor-Gene 3000 (manufactured by Corbett Research, Australia), Rotor-Gene 6000 (manufactured by Qiagen, Germany).
«ALVETSENSE» Real-Time PCR kit method of procedure (cat. No. AL-SARSV-96) is based on the one-step RT-PCR method with real-time detection. The detection of target RNA, as well as control samples of RT-PCR and internal control, is carried out due to the release of fluorophore molecules FAM, ROX and HEX as a result of cleavage of specific probes by polymerase in the amplification process of cDNA strands synthesized from the 2019-nCoV RNA matrix. Registration of the increase or absence of a specific fluorescent signal is performed in each reaction mixture simultaneously using three fluorescence detection channels FAM, ROX and HEX. The kit method of procedure is based on the combination of reverse transcription and amplification in a one-step process carried out in each tube. All the necessary reagents for reverse transcription to obtain the target cDNA and polymerase chain reaction are included in the kit components.