«ALZYME Pfu» DNA polymerase
«ALZYME Pfu» DNA polymerase is designed to synthesize a deoxyribonucleic acid (DNA) molecule by complementary copying of DNA template strands during polymerase chain reaction (PCR) and can be used in the following procedures:
- High-precision PCR.
- Gene cloning.
- DNA synthesis for sequencing.
- Amplification of complex (GC-rich) sequences.
- Long-range PCR.
DNA polymerase «ALZYME Pfu» is an analogue of Phusion High-Fidelity polymerase.
Source: The enzyme is derived from a recombinant E. coli strain carrying a modified Pfu polymerase gene.
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Product | Components |
Reaction buffer for «ALZYME Pfu В» DNA polymerase (c.n. ZPB-047-6.25МL) | Buffer Pfu 10х - 1250 µl x 5 vials |
«ALZYME Pfu» DNA polymerase is a thermostable chimeric enzyme that catalyzes DNA synthesis with 3’→5′ corrective activity and lacks 5’→3′ exonuclease activity. «ALZYME Pfu» carries a DNA affinity domain, which makes the enzyme highly processive and resistant to certain inhibitors.
The enzyme is designed to perform amplification of DNA fragments up to 20,000 bp in length.
«ALZYME Pfu B» reaction buffer provides optimal conditions for the operation of «ALZYME Pfu» DNA polymerase and guarantees increased precision and processivity in the work of this polymerase.
Application field:
«ALZYME Pfu» DNA polymerase is intended for research applications.
- ZP-047-200UN
- ZP-047-500UN
- ZP-047-1KU
«ALZYME Pfu» DNA polymerase (c.n ZP-047-200UN, 200 activity units)
- Pfu DNA polymerase (2 units/µL) – 100 µl x 1 vial;
- Pfu buffer 10х – 1250 µl x 1 vial.
«ALZYME Pfu» DNA polymerase (c.n ZP-047-500UN, 500 activity units)
- Pfu DNA polymerase (2 units/µL) – 250 µl x 1 vial;
- Pfu buffer 10х – 1575 µl x 2 vials.
«ALZYME Pfu» DNA polymerase (c.n ZP-047-1KU, 1000 activity units)
- Pfu DNA polymerase (2 units/µL) – 500 µl x 1 vial;
- Pfu buffer 10х – 1250 µl x 5 vials.