«ALPREP Spin Blood Auto» Reagent kit

«AL­PREP Plas­mid Column» Ex­trac­tion kit is in­tend­ent for auto­mated sim­ul­tan­eous pro­cess­ing of up to 96 samples.

The ex­trac­tion pro­ced­ure in­volves pro­teinase sample treat­ment. Due to the pres­en­ce of hao­trop­ic sub­stances in lys­is and pre­cip­it­at­ing buf­fers, DNA is ad­sorbed on the sur­face of the column silica-gel mem­brane, and then washed off pos­s­ible in­hib­it­ors us­ing wash­ing buf­fers. A series of sub­se­quent washes provides a highly pur­i­fied DNA pre­par­a­tion, which is sub­se­quently be­ing dis­solved in an elut­ing buf­fer and later is used in a PCR.

«AL­PREP Spin Blood Auto» Re­agent kit is de­signed and ad­ap­ted for Qi­aCube auto­mat­ic sta­tion.

«ALPREP Spin Blood Auto» Reagent kit (PS-074A-480, 480 samples):

Part 1:

• Lys­is buf­fer – 30 ml x 2 flasks;
• Pre­cip­it­ant buf­fer con­cen­trate – 2 flasks (60 ml);
• Wash­ing buf­fer con­cen­trate № 1 – 1 flask (196 ml);
• Wash­ing buf­fer con­cen­trate № 2 – 1 flask (132 ml);
• Elu­tion buf­fer – 2 flasks (110 ml).

Part 2:

• Proteinase K (20 mg/ml) – 3 vials (4 ml).

Part 3:

• Extraction plates – 5 pcs.
• Sealing film for plate – 25 pcs.