«ALPREP Spin Blood Auto» Reagent kit
«ALPREP Spin Blood Auto» Reagent kit is designed for automated DNA extraction from blood samples. The operation principle of the kit is based on the automatic DNA purification from blood samples.
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Specimens. Whole blood samples treated with such anticoagulants as citrate, EDTA, and heparin are suitable for extraction. Both fresh and frozen samples can be used. The yield and quality of purified DNA depends on the blood storage conditions.
Analytical characteristics. «ALPREP Spin Blood Auto» Reagent kit provides DNA with purity at least 1.8 according to A260/A280 ratio; the amount of extracted DNA is at least 2-10 μg.
«ALPREP Spin Blood Auto» Reagent kit is designed for automated simultaneous processing of up to 96 samples.
The assay principle is based on the nucleic acids reversible binding to the surface of the column silica gel membrane in the plate due to the presence of chaotropic substances in the precipitation buffer. This is preceded by lysis and proteinase treatment of the sample. A series of subsequent washes ensures the production of a highly purified nucleic acid solution, which is eluted in a low-salt buffer. «ALPREP Spin Blood Auto» Reagent kit is adapted for QiaCube automatic station.
«ALPREP Spin Blood Auto» Reagent kit (PS-074A-480, 480 samples):
Part 1:
- Lysis buffer – 30 ml x 2 bottles;
- Precipitation buffer concentrate – 60 ml × 2 bottles;
- Washing buffer concentrate № 1 – 98 ml × 2 bottles;
- Washing buffer concentrate № 2 – 66 ml × 2 bottles;
- Elution buffer – 110 ml × 2 bottles;
- Extraction plates – 5 pcs;
- Plate sealing film – 25 pcs.
Part 2:
- Proteinase K – 4 ml × 3 tubes.