«ALPREP Spin Blood Auto» Reagent kit
«ALPREP Spin Blood Auto» Reagent kit is designed for obtaining DNA preparations from blood samples. The operation principle of the kit is based on the automatic DNA purification from blood samples.
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Specimens. Whole blood samples treated with such anticoagulants as citrate, EDTA, heparin are suitable for the study. Both fresh and frozen samples can be used. The yield and quality of purified DNA depends on the storage conditions.
Analytical characteristics. «ALPREP Spin Blood Auto» Reagent kit provides DNA extraction with purity (A260/A280 ratio) at least 1.8; the amount of DNA extracted is at least 2-10 mcg.
«ALPREP Plasmid Column» Extraction kit is intendent for automated simultaneous processing of up to 96 samples.
The extraction procedure involves proteinase sample treatment. Due to the presence of haotropic substances in lysis and precipitating buffers, DNA is adsorbed on the surface of the column silica-gel membrane, and then washed off possible inhibitors using washing buffers. A series of subsequent washes provides a highly purified DNA preparation, which is subsequently being dissolved in an eluting buffer and later is used in a PCR.