«ALVETPREP» reagent kit for DNA / RNA isolation
«ALVETPREP» reagent kit (cat. No. AL-PREP2V-96, cat. No. AL-PREP5V-96) is intended for the simultaneous DNA / RNA isolation from biological material for subsequent analysis of the resulting DNA / RNA preparation using polymerase chain reaction (PCR) and polymerase chain reaction with a reverse transcription (RT-PCR).
The functional use of «ALVETPREP» reagent kit (cat. No. AL-PREP2V-96, cat. No. AL-PREP5V-96) is to carry out sample preparation for the subsequent analysis of biological samples, performed in the course of laboratory diagnostics using PCR or RT-PCR. The kit is used to obtain a DNA / RNA preparation free from reverse transcription and PCR inhibitors, which provides high analytical capabilities for subsequent analysis.
Application area. Professional use for research purposes, in specialized laboratories for sanitary examination and the food industry.
Product form and components of «ALVETPREP» reagent kit.
«ALVETPREP» reagent kit is produced in two product forms (variant of a complete kit): – ALVETPREP cat. No. AL-PREP2V-96 – for DNA / RNA extraction in test tubes and plates, ALVETPREP cat. No. AL-PREP5V-96 – for automatic DNA / RNA extraction.
«ALVETPREP» reagent kit (AL-PREP2V-96, for 96 determinations):
- ALVETPREP Magnetic Sorbent – 1,1 mL x 1 tube;
- ALVETPREP Lysis Buffer – 44 mL x 1 vial;
- ALVETPREP Wash Buffer – 40 mL x 2 vials;
- ALVETPREP Elution Buffer – 12 vL x 1 vial.
«ALVETPREP» reagent kit (AL-PREP5V-96, for 96 determinations):
- ALVETPREP Lysis Buffer – 96 x 0,4 mL (1 plate);
- ALVETPREP Wash Buffer – 96 x 0,7 mL (1 plate);
- ALVETPREP Elution Buffer – 96 x 0,1 mL (1 plate);
- Tips for extraction of 96 samples – for 96 samples x 1 plate.
The material for carrying out the DNA / RNA extraction procedure is biological material – smear samples from the external and internal surfaces of food packaging, containers and the products themselves.
Test sample volume – 100 μl.
«ALVETPREP» reagent kit (cat. No. AL-PREP2V-96, cat. No. AL-PREP5V-96) is designed for the DNA / RNA isolation from 96 test samples.
The total time for the manual DNA / RNA isolation procedure from 1 sample is 40 minutes, for automated isolation from 96 samples – 37–45 minutes.
The kit composition and the delivery completeness are shown in tables 1 and 2, respectively. When sampling the test material for the RNA / DNA isolation procedure, it is recommended to use the ALVETMUC sampling reagent kit cat. No. AL-MUC‑V.
Material for identifying the COVID-19 causative agent of food is selected in strict accordance with the following interstate standards: GOST 31904–2012 “Food products. Methods of sampling for microbiological analyses”; GOST R 54354–2011 “Meat and meat products. Organization and methods of microbiological testing”; GOST ISO 22119–2013 “Microbiology of food and animal feeding stuffs. Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions”; GOST R 51426–99 (ISO 6887–83) “Microbiology. Feedstuffs, compound feeds, feed raw materials. General guidance for the preparation of dilutions for microbiological examination”; GOST R 52833–2007 (ISO 22174–2005) “Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) method for the detection of food-borne pathogens. General requirements and definitions”.
Material storage conditions:
- at a temperature from 2 ° С to 8 ° С – within 48 hours;
- at a temperature of minus 20 ° С – within 1 week;
- at a temperature of minus 70 ° C – for a long time.
- Only a single freeze / thaw of the material is permitted.
«ALVETPREP» reagent kit principle of procedure (cat. No. AL-PREP2V-96, cat. No. AL-PREP5V-96) is based on a method that assumes reversible binding of nucleic acid molecules on the surface of magnetic sorbent particles. The test sample is treated with a lysis solution in the presence of magnetic sorbent particles. The result is the destruction of cell membranes, viral envelopes and other biopolymer complexes and the release of nucleic acids. Lysed RNA / DNA binds to the sorbent particles, while other components of the lysed biological material remain in solution and are removed when the sorbent is precipitated on a magnetic stand with subsequent washing of the sorbent. When the elution buffer is added to the magnetic sorbent, DNA is eluted from the sorbent surface into the solution, which is then separated from the sorbent particles by magnetic force. The result of the above procedure is a highly purified DNA preparation free from inhibitors of the amplification reaction, which provides a high analytical sensitivity of the PCR research.
The kit application is possible only by personnel trained in molecular diagnostics methods and the work rules in a clinical diagnostic laboratory.