Description
PURPOSE
«ALMUNO Antibiotic Tetracycline» Kit is intended for quantitative determination of tetracyclines by ELISA in the following matrices:
- milk (raw, pasteurized, sterilized), cream, reconstituted milk powder, dairy desserts, butter, condensed milk, ice cream, milk-based shakes (including yoghurt), reconstituted milk powder for infant food;
- cheese, cottage cheese and cottage cheese products, fermented milk products (ayran, yoghurt), sour cream, buttermilk, whey, reconstituted whey powder;
- meat, meat products, ready-made meat products, semi-finished meat and meat-growing products, canned meat and meat-growing products, lard, bacon, animal fats, by-products;
- fish, processed and unprocessed fish products of animal origin, including for infant food (culinary products and semi-finished fish products, canned and preserved fish, caviar, cooked and frozen fish products), shrimps;
- eggs, egg powder;
- honey.
PRINCIPLE OF METHOD
The method is based on a competitive colourimetric enzyme immunoassay. During the assay, tetracycline in the sample competes with the antibiotic immobilised on the surface of the microplate wells for binding to antibodies to tetracycline. The resulting immunocomplexes are detected using horseradish peroxidase-conjugated secondary antibodies. The concentration of tetracycline in the sample is determined from a calibration curve constructed using 6 calibration solutions.
DETECTION LIMITS & MEASURMENT RANGES
| Matrix | Measuring range, µg/kg (µg/L) |
|
Milk (raw, pasteurised, sterilised, ultra-pasteurised), cream, fermented milk products (ryazhenka, ayran, sour milk products, cottage cheese, kefir, acidophilus with or without fillers), cottage cheese products. |
1,0-80,0 |
|
Dairy desserts, dairy-based shakes (including yoghurt), buttermilk, fermented products (yoghurt,arenyets, sour cream) milk powder, whey, whey powder, milk powder for infant food, cheese, condensed milk, dairy-based ice cream, eggs, egg powder |
|
|
Meat, ready-to-eat meat products, lard including bacon, animal fats, animal by-products, tinned meat and meat andegetable products, semi-finished meat and preserves, caviar |
|
|
Processed and unprocessed fish products of animal origin, including for infant food (fish, cooked and semi-finished fish products, cooked and frozen fish products), shrimps |
|
| butter | 3,0-160,0 |
| honey | 3,0-80,0 |
BENEFITS
- low quantification limit – 1 µg/kg for dairy, meat and fish products, 3 µg/kg for butter and honey;
- a possibility to perform ELISA with more than six strips at the same time;
- software in the form of a template for automatic calculation of results;
- shelf-life when stored properly: 18 months.
