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«ELISA antibiotic tetracycline» reagent kit
Item code: 1016-04EA
«ELISA antibiotic tetracycline» reagent kit kit is used to determine tetracycline in the following matrices:
- Milk (raw, pasteurized, sterilized).
- Milk powder.
- Butter.
- Cheese.
- Sour cream.
- Kefir.
- Yogurt.
- Cottage cheese.
- Milk whey.
- Reconstituted milk powder infant formula.
- Reconstituted powdered milk whey.
- Meat.
- Meat products.
- Eggs.
- Honey.
- Fish & shrimps.
Use primary filter 450 nm.
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Key Benefits
Kit Content
Measuring detection limits
Key Benefits
Description
- Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards).
- All unused wells can be stored with the desiccant sealed in the foil package.
- Storage temperature: 2–8 °С.
- Shelf-life when stored properly: 12 months.
Kit Content
«ELISA antibiotic tetracycline» reagent kit (1016-04EA, 96 determinations or testing of 42 samples in duplicate).
- Microtiter-plate (12 strips with 8 wells each) – 1 pc;
- Конъюгат – 16 cm3;
- Tetracycline (lyophilized) – 3 pcs;
- Empty test tubes for standards solutions (0 ng/cm3, 0,05 ng/cm3, 0,15 ng/cm3, 0,40 ng/cm3, 0,80 ng/cm3, 1,6 ng/cm3) – 6 pcs;
- Tetracycline antibody solution – 12 cm3;
- Standard diluent – 30 cm3;
- 5x Extraction buffer (concentrated) * – 55 cm3;
- Sample balance buffer (concentrated) * – 1,5 cm3;
- 10x Sample diluent buffer (concentrated) * – 10 cm3;
- 20x Wash buffer (concentrated) * – 28 cm3;
- TMB Substrate * – 12 cm3;
- Stop reagent * – 12 cm3;
- Lyophilized sample with high concentration of tetracycline (150 ng) – 1 pc.
Components marked with * are interchangeable within their shelf-life if series number coincides.
Measuring detection limits
| Matrix | Detection limit | Range |
| Milk, cream, Reconstituted powdered milk, milk-based desserts, condensed milk, milk-based ice cream, milk-based smoothies, reconstituted powdered milk mixture for infant feeding | 1 µg/kg (µg/l) | 1 – 16 µg/kg (µg/l) |
| Cheese, cottage cheese and curd products, fermented milk products (ryazhenka, ayran, curdled milk, varenets, acidophilus), sour cream, buttermilk, milk whey, reconstituted powdered milk whey | 1 µg/kg (µg/l) | 1 – 16 µg/kg (µg/l) |
| Meat, meat products, ready-made meat products, semi-finished meat and meat-growing products, canned meat and meat-growing products, salo, speck, animal fats, by-products | 1 µg/kg (µg/l) | 1 – 16 µg/kg (µg/l) |
| Fish and unprocessed fish products of animal origin (culinary products and semi-finished fish products, canned fish and preserves, caviar, cooked and frozen fish products), shrimp | 1 µg/kg (µg/l) | 1 – 16 µg/kg (µg/l) |
| Eggs, egg powder, honey | 1 µg/kg (µg/l) | 1 – 16 µg/kg (µg/l) |
| Butter | 3 µg/kg (µg/l) | 3 – 32 µg/kg (µg/l) |
| Blood serum | 3 µg/kg (µg/l) | 3 – 64 µg/kg (µg/l) |