«ALPREP-2» Extraction Kit
«ALPREP-2» Extraction Kit is based on the reversible binding of nucleic acid molecules with the surface of magnetic beads. The sample is treated with a lysis buffer in the presence of magnetic beads. As a result, cell membranes, viral membranes and other biopolymer complexes are destroyed and nucleic acids are released. Dissolved nucleic acid binds to the magnetic beads, while other components of the lysed biological material remain in the solution and are removed after magnetic precipitation on a magnetic rack followed by several washing steps. When an elution buffer is added to magnetic beads, nucleic acids are eluted from the surface of the magnetic beads into solution, which is then separated from the beads by magnetic force. As a result of this procedure, a highly purified DNA (RNA) sample is obtained, free of PCR-inhibitors, which ensures high analytical sensitivity of the PCR study.
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Analytical characteristics
- Genomic DNA and RNA, including human, animal.
- Pasmid/bacterial DNA.
- Viral DNA and RNA.
- Animal DNA and RNA.
- PCR products (amplicons).
- cDNA.
Video instruction for using the kit «ALPREP-2»
«ALPREP-2» Extraction Kit provides the preanalytical stage for the biological samples subsequent analysis, performed during the clinical laboratory diagnostics of human infectious pathology using PCR or RT-PCR. The kit allows to obtain inhibitors-free DNA/RNA samples, which provides high analytical capabilities for subsequent analysis.
Field of application. Professional use for research purposes, in specialized medical & healthcare institutions.
Specimens. Extraction procedure is performed for biological material (clinical samples), including swabs, scrapings, sputum, white blood cells, urine (sediment), ejaculate, prostatic fluid (100 to 106 cells per sample). The volume of the test sample is 100 μl.
The total time of the protocol (DNA/RNA extraction from the single sample):
- 40 minutes (manual)
- 35 minutes per 96 samples (automatic station)
«ALPREP-2» Extraction Kit (AL-PREP2-96):
- AlgimedPREP magnetic beads – 1.1 mL x 1 tube;
- AlgimedPREP lysis buffer – 44 mL 1 x vial;
- AlgimedPREP wash buffer #1 – 40 mL x 2 vials;
- AlgimedPREP wash buffer #2 – 40 mL x 2 vials;
- AlgimedPREP elution buffer – 12 mL x 1 tube;
- AlgimedPREP storage buffer – 1.8 mL x 1 tube.