«ALPREP Forensic Plus» Extraction Kit
«ALPREP Forensic Plus» Extraction Kit is designed to extract DNA from most types of forensic samples for subsequent PCR analysis.
Professional use for research purposes, in specialized laboratories of forensic examination institutions / forensic organizations.
- dry and wet blood stains, semen, buccal and skin epithelial cells or tissues collected on cotton buds, paper and other media;
- fragments of hair with bulbs;
- nail plates;
- subungual contents;
- soft tissues (including formalin-fixed and paraffin-embedded);
- epithelial and sperm fractions (after differential lysis);
- adhesive materials (adhesive tapes, postage stamps, envelope flaps, cigarette butts, chewing gum);
- blood on soil particles;
- bone samples.
«ALPREP Forensic Plus» Extraction Kit is designed to extract DNA from 100 biological samples.
PDF for download
The kits are certified for the absence of foreign DNA/RNA of various etiologies, the absence of DNases/RNases in a testing laboratory certified according to ISO 17025.
|Reaction buffer for ALPREP BTA lysis buffer (for 100 samples)||50 ml x 1 bottle|
«ALPREP Forensic Plus» Extraction Kit is based on the reversible binding of nucleic acid molecules on the surface of magnetic sorbent particles.
Sample is treated with a lysing solution. As a result, cell membranes, biopolymer complexes are destroyed and nucleic acids are released.
At the stage of sorption, the dissolved DNA binds to the sorbent particles, while other components of the lysed biological material remain in solution and then removed during the deposition of the sorbent on a magnetic stand, followed by washing of the sorbent. When the elution buffer is added to the magnetic sorbent, DNA is eluted from the surface of the sorbent into a solution, which is then separated from the sorbent particles by magnetic force. As a result of this procedure, a highly purified DNA is obtained, free from inhibitors, which ensures high analytical sensitivity of the PCR.