«ALZYME K» proteinase
«ALZYME K» proteinase is intended for purification of enzyme mixtures and cell lysates from unwanted proteins during RNA/DNA isolation from microorganisms, cell cultures, plants and other samples, for nucleases inactivation and increasing the tissue lysis efficiency during nucleic acid isolation.
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molecular and cell biology, immunohistochemistry and other fields of biology and chemistry for various proteins cleavage, including preparation of chromosomal DNA for electrophoresis, western blotting and nuclease removal in DNA and RNA preparations.
- Cleaves peptide bonds from the carboxyl group side of aromatic, aliphatic and hydrophobic amino acids.
- The enzyme is stable in urea, SDS, EDTA and has the ability to degrade natural proteins.
- The enzyme is used to reduce RNase and DNAase activity and purify enzyme mixtures/cell lysates from protein impurities of various nature.
- It retains activity in a wide pH range (optimally 6.5-9.5) and relatively high temperatures (optimally 50-65 °C), functions in the presence of chaotropic salts, detergents and at high temperatures. To inactivate the enzyme, the reaction mixture is incubated at 95 °C for 10 minutes.