«ALzyme-RT» reverse transcriptase
ALzyme-RT reverse transcriptase is designed to synthesize the first cDNA strand from an RNA matrix and can be used in the following procedures:
- Synthesis of the first cDNA strand for PCR and real-time PCR.
- cDNA synthesis for subsequent cloning.
- Creation of cDNA libraries.
- Incorporation of labeled nucleotides in the cDNA strand.
Source: The enzyme was obtained from a recombinant E. coli strain expressing a modified FeLV (Feline Leukemia Virus) revertase gene.
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| Product | Components |
| Reaction buffer for ALzyme-RT-В reverse transcriptase (c.n. ZRT-B) | 1250 µl x 1 vial |
| Dilution buffer for ALzyme-RT-DB reverse transcriptase (c.n. ZRT-DB) | 1250 µl x 1 vial |
«ALzyme-RT» reverse transcriptase is a highly purified recombinant enzyme designed for reverse transcription. The enzyme is an alternative to MMLV reverse transcriptase with several advantages: it has increased thermostability and processivity.
Allows the synthesis of cDNA fragments up to 12000 bp in length. The optimal working temperature is 50 ℃. Inactivation conditions: 80℃ for 10 minutes. Incubation (synthesis) time depends on the matrix and varies from 10 seconds to 60 minutes.
The properties of ALzyme-RT reverse transcriptase are similar to FeLV, which is more precise in nature than MMLV-RT.
ALzyme-RT reverse transcriptase contains mutations that further enhance thermostability and accuracy.
ALzyme-RT-B reaction buffer provides optimal conditions for ALzyme-RT reverse transcriptase.
ALzyme-RT reverse transcriptase is suitable for performing single tube RT-PCR using ALzyme-Taq.
Application field:
«ALzyme-RT» reverse transcriptase is intended for research purposes.
- ZRT-100
- ZRT-500
«ALzyme-RT» reverse transcriptase (20 000 units), cat. number ZRT-100
- ALzyme-RT reverse transcriptase (200 units/µL), 100 µl x 1 vial;
- ALzyme-RT-В buffer, 1250 µl x 2 vials.
«ALzyme-RT» reverse transcriptase (100 000 units), cat. number ZRT-500
- ALzyme-RT reverse transcriptase (200 units/µl), 500 µl x 1 vial;
- ALzyme-RT-В buffer, 1250 µl x 10 vials.