«ALZYME RT» reverse transcriptase
«ALZYME RT» reverse transcriptase is designed to synthesize the first cDNA strand from an RNA matrix and can be used in the following procedures:
- Synthesis of the first cDNA strand for PCR and real-time PCR.
- cDNA synthesis for subsequent cloning.
- Creation of cDNA libraries.
- Incorporation of labeled nucleotides in the cDNA strand.
Source: The enzyme was obtained from a recombinant E. coli strain expressing a modified FeLV (Feline Leukemia Virus) revertase gene.
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| Product | Components |
| Reaction buffer for ALZYME RT В reverse transcriptase (c.n. ZRB-043-6.25МL) | 1250 µl x 1 vial |
| Dilution buffer for ALZYME RT DB reverse transcriptase (c.n. ZRD-043-6.25МL) | 1250 µl x 1 vial |
«ALZYME RT» reverse transcriptase is a highly purified recombinant enzyme designed for reverse transcription. The enzyme is an alternative to MMLV reverse transcriptase with several advantages: it has increased thermostability and processivity.
Allows the synthesis of cDNA fragments up to 12000 bp in length. The optimal working temperature is 50 ℃. Inactivation conditions: 80℃ for 10 minutes. Incubation (synthesis) time depends on the matrix and varies from 10 seconds to 60 minutes.
The properties of ALZYME RT reverse transcriptase are similar to FeLV, which is more precise in nature than MMLV-RT.
ALZYME RT reverse transcriptase contains mutations that further enhance thermostability and accuracy. ALZYME RT B reaction buffer provides optimal conditions for ALZYME RT reverse transcriptase. ALZYME RT reverse transcriptase is suitable for performing single tube RT-PCR using ALZYME Taq.
Application field:
«ALZYME RT» reverse transcriptase is intended for research purposes.
- ZR-043-20KU
- ZR-043-100KU
«ALZYME RT» reverse transcriptase (20 000 units), cat. number ZR-043-20KU
- ALZYME RT reverse transcriptase (200 units/µL), 100 µl x 1 vial;
- ALZYME RT В buffer, 1250 µl x 2 vials.
«ALZYME RT» reverse transcriptase (100 000 units), cat. number ZR-043-100KU
- ALZYME RT reverse transcriptase (200 units/µl), 500 µl x 1 vial;
- ALZYME RT В buffer, 1250 µl x 10 vials.