«ALG-CASEIN A1/A2 MAX» reagent kit
«ALG-CASEIN A1/A2 MAX» – complete reagent kit, includes reagents for all stages of the study: samples prelysis, DNA extraction and polymerase chain reaction (PCR). The reagent kit is designed to study 96 samples.
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The reagent kit is designed to identify the alleles A1 and A2 of the gene β-casein of cattle in the material of cartilage, blood, sperm and whole milk samples by PCR with real-time fluorescent detection, and to identify the percentage of alleles in whole milk samples. «ALG-CASEIN A1/A2 MAX» reagent kit includes all the necessary components for real-time PCR: positive, negative and internal control, water, buffers and enzymes.
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We express our gratitude for the joint development and validation of studies on animal samples to the employees of the Center for Genetics and Reproduction (a branch of Algimed Techno LLC). Services for genotyping of cattle to identify A1 and A2 animals can be ordered on the website – https://labgen.by/.
The test principle is based on DNA extraction from material samples and subsequent amplification of a specific DNA site with allele-specific probes. The accumulation of specific amplification products is recorded by measuring the intensity of the fluorescent signal in real time using the FAM and HEX channels in real time.
- Low cost;
- A turnkey solution that includes reagents for all stages of PCR analysis;
- Time input reduction (automation of DNA extraction stage);
- Fast, reliable and highly sensitive result;
- Easy result analysis;
- Analysis of A1 and A2 alleles percentage in whole milk.
Research material
- Bos taurus tissue samples (blood, cartilage tissue);
- Whole milk;
- Bos taurus semen.
Time of the research
- DNA extraction: from 30 minutes;
- PCR analysis:
- time for manual manipulations – 5 – 15 min;
- PCR protocol time – 71 min;
- total time for analysis – 90 min.
Detection threshold:
- 7,5 genomic copies per reaction;
- detection of up to 1 individual carrier of the A1 allele out of 50 in pooled milk samples.
Shelf life: 12 months.
- CASMAX‑1
- CASMAX‑2
«ALG-CASEIN A1/A2 MAX» reagent kit (release form 1, 96 reactions):
Part 1
- Magnetic Sorbent 1,1 ml × 2 pcs;
- Prelysis Buffer 52,5 ml × 1 pcs;
- Sorbing Buffer 21 ml × 1 pcs;
- Lysing Buffer 44 ml × 1 pcs;
- Wash Buffer No. 1 50 ml × 1 pcs;
- Wash Buffer No. 2 50 ml × 1 pcs;
- Elution Buffer 12 ml × 1 pcs;
- Dilution Buffer 12 ml × 1 pcs.
Part 2
- А1А2 Casein PCR Buffer (2х) 1,25 ml × 1 pcs;
- PCR Water 1,25 ml × 1 pcs;
- А1А1 Positive Control Sample 100 μl × 1 pcs;
- А1А2 Positive Control Sample 100 μl × 1 pcs;
- А2А2 Positive Control Sample 100 μl × 1 pcs;
- Negative Control Sample 100 μl × 1 pcs.
«ALG-CASEIN A1/A2 MAX» reagent kit (release form 2, 96 reactions):
Part 1
- Magnetic Sorbent 1,1 ml × 1 pcs;
- Prelysis Buffer 52,5 ml × 1 pcs;
- Sorbing Buffer 21 ml × 1 pcs;
- Lysing Buffer 96 x 0,4 ml × 1 pcs;
- Wash Buffer No. 1 96 x 0,7 ml × 1 pcs;
- Wash Buffer No. 2 96 x 0,7 ml × 1 pcs;
- Elution Buffer 96 x 0,1 ml × 1 pcs;
- Dilution Buffer 12 ml × 1 pcs.
Part 2
- А1А2 Casein PCR Buffer (2х) 1,25 ml × 1 pcs;
- PCR Water 1,25 ml × 1 pcs;
- А1А1 Positive Control Sample 100 μl × 1 pcs;
- А1А2 Positive Control Sample 100 μl × 1 pcs;
- А2А2 Positive Control Sample 100 μl × 1 pcs;
- Negative Control Sample 250 μl × 1 pcs.