«ELISA-IgM-Sars-CoV‑2» reagent kit
«ELISA-IgM-Sars-CoV‑2» reagent kit created to detect immunoglobulins M by ELISA method. The Kit is confgured to analyze 46 unknown samples in negative control triplicate, positive control monoplicate. 96 defnitions in total.
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In Vitro diagnostic ELISA based test kit for immunoglobulins M to SARS-Cov-2 in serum and plasma (EDTA, heparin, ACD or citrate).
Immunoglobulin M (IgM) is the largest antibody. IgM represnts approximately 5-10% of the human blood plasma antibodies. It is the first antibody to appear when the hunman body figts a new infection.
The IgM detection may assist to determine immune response in patients with an early stage of coronavirus disease.
How to determine antibodies to the SARS-CoV‑2 coronavirus
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The principle is based on indirect non-competitive ELISA analysis. The study sample and control sample are incubated in the wells coated with arecombinant nucleocapsic protein. Immunoglobulins to this protein when presentin the sample bind specifically to the protein immobilized in the wells. Next, IgM- class antibodies are detected with the help of a second antibody in aperoxidase conjugate. After incubation with the substrate and stop solution application, the optical density of the sample is measured at 450 nm. The measuredoptical density is directly related to the number of IgM in the tested and control samples.
| Kit components | Quantity |
|---|---|
| Immunoadsorbent | 1 pce |
| Conjugate | 11 ml |
| Positive Control | 0,5 ml |
| Negative Control | 1 ml |
| Sample Diluent | 13 ml |
| Wash Concentrate(20x) | 25 ml |
| TMB Substrate | 12 ml |
| Stop Solution | 12 ml |
Components marked with * are interchaµgeable within their shelf-life if series number coincides.