«ALMIR» reagent kits
The kit implements the original technology of two-way priming of reverse transcription and PCR with two micro-RNA-specific primers (Androvic et al., 2017), which provides highest analysis accuracy. Optimization of the RT-primer structure and detection of amplification using a probe ensures reliable results and higher sensitivity of the assay compared to other technologies (Korobkina et al., 2018). This allows working with low concentration RNA samples.
ОТ-RT-PCR is the primary method of microRNA analysis in laboratory practice due to its reliability and cost-effectiveness.
PDF for download
«ALMIR» reagent kits available for order
| Title and ID |
 |
 |
 |
|---|---|---|---|
|
hsa-miR-106b-5p
MIMAT0000680 |
|||
|
hsa-miR-126-3p
MIMAT0000445 |
|||
|
hsa-miR-145-5p
MIMAT0000437 |
|||
|
hsa-miR-196b-5p
MIMAT0001080 |
|||
|
hsa-miR-10b-5p
MIMAT0000254 |
|||
|
hsa-miR-31-5p
MIMAT0000089 |
|||
|
hsa-miR-221-3p
MIMAT0000278 |
|||
|
hsa-miR-200a-3p
MIMAT0000682 |
|||
|
hsa-miR-200b-3p
MIMAT0000318 |
|||
|
hsa-miR-200c-3p
MIMAT0000617 |
|||
|
hsa-miR-10a-5p
MIMAT0000253 |
|||
|
hsa-miR-195-5p
MIMAT0000461 |
|||
|
hsa-miR-181b-5p
MIMAT0000257 |
|||
|
hsa-miR-191-5p
MIMAT0000440 |
|||
|
hsa-miR-1246-5p
MIMAT0005898 |
|||
|
hsa-miR-375-3p
MIMAT0000728 |
|||
|
hsa-miR-16-5p
MIMAT0000069 |
|||
|
hsa-miR-29b-3p
MIMAT0000100 |
|||
|
hsa-miR-21-5p
MIMAT0000076 |
|||
|
hsa-miR-451a-5p
MIMAT0001631 |
|||
|
hsa-miR-182-5p
MIMAT0000259 |
|||
|
hsa-miR-205-5p
MIMAT0000266 |
|||
|
hsa-miR-30a-5p
MIMAT0000087 |
|||
|
hsa-miR-371-3p
MIMAT0000723 |
|||
|
hsa-miR-371-5p
MIMAT0004687 |
|||
|
hsa-miR-185-5p
MIMAT0000455 |
|||
|
hsa-miR-7-5p
MIMAT0000252 |
|||
|
hsa-miR-15a-5p
MIMAT0000068 |
|||
|
hsa-miR-20a-5p
MIMAT0000075 |
|||
|
hsa-miR-23a-3p
MIMAT0000078 |
|||
|
hsa-miR-24-3p
MIMAT0000080 |
|||
|
hsa-miR-26b-5p
MIMAT0000083 |
|||
|
hsa-miR-27a-3p
MIMAT0000084 |
|||
|
hsa-miR-34a-5p
MIMAT0000255 |
|||
|
hsa-miR-93-5p
MIMAT0000093 |
|||
|
hsa-miR-125b-5p
MIMAT0000423 |
|||
|
hsa-miR-141-3p
MIMAT0000432 |
|||
|
hsa-miR-143-3p
MIMAT0000435 |
|||
|
hsa-miR-146b-5p
MIMAT0002809 |
|||
|
hsa-miR-155-5p
MIMAT0000646 |
|||
|
hsa-miR-186-5p
MIMAT0000456 |
|||
|
hsa-miR-302a-3p
MIMAT0000684 |
|||
|
hsa-miR-302b-3p
MIMAT0000715 |
Normal |
||
|
hsa-miR-302c-3p
MIMAT0000717 |
|||
|
hsa-miR-302d-3p
MIMAT0000718 |
Normal |
||
|
hsa-miR-367-3p
MIMAT0000719 |
Normal |
||
|
hsa-miR-372-3p
MIMAT0000724 |
Normal |
||
|
hsa-miR-373-3p
MIMAT0000726 |
|||
|
hsa-miR-429-3p
MIMAT0001536 |
|||
|
hsa-miR-495-3p
MIMAT0002817 |
|||
|
hsa-miR-597-5p
MIMAT0003265 |
Normal |
||
|
hsa-miR-1290-3p
MIMAT0005880 |
|||
|
hsa-miR-3688-3p
MIMAT0018116 |
Normal |
Cancer |
|
|
cel-miR-39-3p
MIMAT0000010 |
Normal |
||
|
SNORD48
SNORD48 |
miRBase |
Normal |
|
|
SNORD38B
SNORD38B |
miRBase |
Normal |
|
|
hsa-let-7a-5p
MIMAT0000062 |
|||
|
hsa-miR-29c-3p
MIMAT0000681 |
|||
|
hsa-miR-146a-5p
MIMAT0000449 |
|||
|
hsa-miR-192-5p
MIMAT0000222 |
|||
|
hsa-miR-216a-5p
MIMAT0000273 |
|||
|
hsa-miR-217-5p
MIMAT0000274 |
|||
For commercial proposal or technical support please send your inquiry to sales@algimed-techno.com.
«ALMIR» reagent kits:
- Optimized enzyme mixtures for RT and PCR.
- Balanced oligonucleotide solutions for the analysis of a specific miRNA molecule: RT primer, a pair of PCR primers and a fluorescently labeled probe for amplification detection.
- Set of eight calibrators – serial dilutions of a synthetic analogue of the detected miRNA molecule.
2.5 – 3 hours, including RT (45′) and PCR (60′).
Material requirements
RNA must be isolated (from cells, tissues, biological fluids) by methods that exclude the «loss» of short molecules.