«ALZYME Taq» DNA polymerase
«ALZYME Taq» DNA polymerase is designed to synthesize a deoxyribonucleic acid (DNA) molecule by complementary copying of DNA template strands during the polymerase chain reaction (PCR). «ALzyme-Taq» DNA polymeraseis intended for research purposes.
«ALZYME HSTaq» DNA polymerase is used for:
- Traditional PCR.
- Real-time PCR with TaqMan probes, intercalating dyes.
- Fragments amplification for TA-cloning.
- Incorporation of modified nucleotides in DNA.
Source: The enzyme is derived from a recombinant E. coli strain carrying a modified Taq polymerase gene.
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| Product | Components |
| Buffer «ALZYME Taq BM» (c.n. ZTB-061-M-6.25МL) | Buffer Taq 10х - 1250 µl x 5 vials |
| Buffer «ALZYME Taq BM free», without Mg++ (c.n. ZTB-061-6.25МL) | Buffer Taq Mg free 10х - 1250 µl x 5 vials |
| Solution MgCl2 «ALZYME M» (c.n. ZM-061-6.25МL) | Solution MgCl2 50 mM - 1250 µl x 5 vials |
«ALZYME Taq» is a recombinant heat resisting enzyme that catalyzes 5’→3′ single-stranded matrix DNA synthesis in the presence of dNTPs and primers. It has 5’→3′ exonuclease activity, but does not exhibit corrective activity. «ALZYME Taq» allows efficient amplification of DNA fragments up to 5 bp. Specific mutations make the enzyme resistant to certain inhibitors contained in blood, soil, plant tissue, etc. In addition, «ALZYME Taq» has deoxynucleotidyl transferase activity, so that a significant portion of the amplified DNA molecules carry protruding deoxyadenosine (dA) residues at the 3′-end. The enzyme concentration is 5 units/µl.
- ZT-061-М-500UN
- ZT-061-М-2.5KU
- ZT-061-500UN
- ZT-061-2.5KU
«ALZYME Taq» DNA polymerase, 10х buffer with MgCl2 (500 units).
- ALZYME Taq (5 units/µL) – 100 µL x 1 vial;
- Buffer Taq 10х – 1250 µL x 1 vial.
«ALZYME Taq» DNA polymerase, 10х buffer with MgCl2 (2500 units).
- ALZYME Taq (5 units/µL) – 500 µL x 1 vial;
- Buffer Taq 10х – 1250 µL x 5 vials.
«ALZYME Taq» DNA polymerase, 10х buffer without MgCl2 (500 units).
- ALZYME Taq (5 units/µL) – 100 µL x 1 vial;
- Buffer Taq Mg free 10х – 1250 µL x 1 vial;
- Solution MgCl2 50 mM – 1250 µL x 1 vial.
«ALZYME Taq» DNA polymerase, 10х buffer without MgCl2 (2500 units).
- ALZYME Taq (5 units/µL) – 500 µL x 1 vial;
- Buffer Taq Mg free 10х – 1250 µL x 5 vials;
- Solution MgCl2 50 mM – 1250 µL x 5 vials.