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«ALMASS Neo» reagent kit
«ALMASS Neo» reagent kit for the detection of Congenital Metabolic Disease by HPLC-MS/MS.
Neonatal screening is the newborn screening program for genetic disorders. Hereditary metabolic diseases involve the quantitative analysis of a number of markers. The procedure begins in the maternity hospital, where five drops of blood are drawn from the newborn, applying them to a special card, which is then dried. This produces a sample – a Dried Blood Spot – which will be sent for further analysis. Abnormal marker analytes will indicate metabolic disorders and allow the identification of potentially sick newborns. The panel of analytes is represented by amino acids and acylcarnitines, with adenosine also added to the expanded panel.
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Screening is not literally an «assay by which a diagnosis can be made quickly and unambiguously». Further examinations are performed by other specialists, additional tests are prescribed, etc.
The analysis is performed by tandem mass spectrometry, with the triple quadrupole being the best instrument here. When working with a kit without a derivatizing agent, a mixture of isotope-labeled internal standards is used. The operator adds them at the earliest stage, as expected, and after sample preparation puts them into the autosampler of the instrument. The analysis is done without a column, by Flow Injection. The mobile phase picks up the injected volume, and the moment the mixture enters the detector, the flow is abruptly dumped and pulled by a slow current. The absence of a column greatly reduces the analysis time. With proper setup and timely maintenance of the instrument, the technique is simple and quick. And this is the key in the context of a mass study.
«ALMASS Neo» reagent kit (for 960 tests):
- Internal Standard – 2 pce;
- Control 1 (high) – 2 pce;
- Microplate – 2 pce;
- Control 2 (low) – 20 pce;
- Adhesive microplate covers – 20 pce.
«ALMASS Neo» Assay Solutions (for 960 tests):
- «ALMASS Neo» Flow solwent – 800 mL;
- «ALMASS Neo» Extraction solution – 360 mL.