«ALSENSE Phytoplasma mali RT-PCR» reagent kit
«ALSENSE Phytoplasma mali RT-PCR» reagent kit for the detection of Phytoplasma mali by RT-PCR method is designed for the detection of specific Phytoplasma mali DNA by PCR using TaqMan technology in nucleic acid preparations.
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Application field: The kit is suitable for research purposes.
The ALSENSE reagent kit for the detection of Phytoplasma mali by RT-PCR is based on a real-time polymerase chain reaction (RT-PCR) with hybridization-fluorescence detection on a DNA matrix. The PCR method is based on the multiple doubling of a specific DNA region with DNA polymerase under artificial conditions (in vitro). The amplification target is the 16S ribosomal RNA gene region of phytoplasmas of the 16SrX group. An internal control (ICS) was designed based on the 18S apple ribosomal RNA gene sequences.
The TaqMan probe to the Candidatus Phytoplasma mali genome region carries a FAM dye tag at the 5′-end. The ICS-specific probe is labeled with the HEX fluorophore, allowing the user to validate the DNA extraction step from plants.