«ALSENSE Erwinia amylovora qPCR kit»
«ALSENSE Erwinia amylovora qPCR kit» for the detection of Erwinia amylovora by qPCR method is designed for the detection of specific Erwinia amylovora DNA by PCR using TaqMan technology in nucleic acid preparations.
Erwinia amylovora is the causative agent of bacterial blight, the most dangerous necrotic plant disease of the Rosaceae family. Rapid spreading of the bacteria through the tissues of the host can cause the death of entire trees during a single growing season. Infestation of flowers by E. amylovora can significantly reduce yield and marketability. The phytopathogen is a quarantine object for the Republic of Belarus, countries of the Eurasian Economic Union and countries of the European and Mediterranean Plant Protection Organization (EPPO A2 list).
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Application field: The kit is suitable for research purposes.
An example of a positive PCR result using «ALSENSE Erwinia amylovora qPCR kit».
«ALSENSE Erwinia amylovora qPCR kit» is based on the recommendations of the European and Mediterranean Plant Protection Organization (EPPO) and allows the detection of pathogen DNA by real time polymerase chain reaction. The amplification target is the ams operon site of Erwinia amylovora. An internal control (IC) was designed based on the 18S ribosomal RNA gene sequences of apple and pear trees.
The TaqMan® probe to the Erwinia amylovora genome site carries a FAM dye label at the 5′-end. The ICS-specific probe is labeled with the HEX fluorophore, allowing the user to validate the DNA extraction step from plants.
Analytical specificity: 100%
Analytical sensitivity: To test the analytical sensitivity of the ALSENSE diagnostic kit for the detection of Erwinia amylovora by qPCR, a series of 8 ten-fold dilutions of DNA obtained from an infected plant with mild symptoms of bacterial blight was performed. A stable positive reaction was observed when the original sample was diluted 107 times.
- SEA-075–24
- SEA-075–96
«ALSENSE Erwinia amylovora qPCR kit» (SEA-075–24, 24 tests):
- 2x PCR mastermix ERW – 1 vial (300 ml);
- Positive Control ERW – 1 vial (15 µl);
- Negative Control – 1 vial (15 µl).