«ALZYME HSTaq» DNA polymerase
«ALZYME HSTaq» DNA polymerase is designed to synthesize a deoxyribonucleic acid (DNA) molecule by complementary copying of DNA template strands during the polymerase chain reaction (PCR). «ALZYME HSTaq» DNA polymerase is intended for research purposes.
«ALZYME HSTaq» DNA polymerase is used for:
- Traditional PCR.
- Real-time PCR with TaqMan probes, intercalating dyes.
- Amplification of fragments for TA-cloning.
- Incorporation of modified nucleotides in DNA.
Source: The enzyme is derived from a recombinant E. coli strain carrying a modified Taq polymerase gene.
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| Product | Components |
| Buffer «ALZYME Taq BM» (c.n. ZTB-061-M-6.25МL) | Buffer Taq 10х - 1250 µl x 5 vials |
| Buffer «ALZYME Taq BM free», without Mg++ (c.n. ZTB-061-6.25МL) | Buffer Taq Mg free 10х - 1250 µl x 5 vials |
| Solution MgCl2 «ALZYME M» (c.n. ZM-061-6.25МL) | Solution MgCl2 50 mM - 1250 µl x 5 vials |
«ALZYME HSTaq» is a recombinant heat resisting enzyme that catalyzes 5’→3′ single-stranded matrix DNA synthesis in the presence of dNTPs and primers. It has 5’→3′ exonuclease activity, but does not exhibit corrective activity. «ALZYME HSTaq» allows efficient amplification of DNA fragments up to 5 bp. Specific mutations make the enzyme resistant to certain inhibitors contained in blood, soil, plant tissue, etc. In addition, «ALZYME HSTaq» has deoxynucleotidyl transferase activity, so that a significant portion of the amplified DNA molecules carry protruding deoxyadenosine (dA) residues at the 3′-end. The enzyme concentration is 5 units/µl.
«Hot Start» technology. The enzyme is not active under PCR mixture mixing conditions and is activated after the initial denaturation step.
- ZH-061-М-500UN
- ZH-061-М-2.5KU
- ZH-061-500UN
- ZH-061-2.5KU
«ALZYME HSTaq» DNA polymerase, 10х buffer with MgCl2 (500 units).
- ALZYME HSTaq (5 units/µL) – 100 µL x 1 vial;
- Buffer Taq 10х – 1250 µL x 1 vial.
«ALZYME HSTaq» DNA polymerase, 10х buffer with MgCl2 (2500 units).
- ALZYME HSTaq (5 units/µL) – 500 µL x 1 vial;
- Buffer Taq 10х – 1250 µL x 5 vials.
«ALZYME HSTaq» DNA polymerase, 10х buffer without MgCl2 (500 units).
- ALZYME HSTaq (5 units/µL) – 100 µL x 1 vial;
- Buffer Taq Mg free 10х – 1250 µL x 1 vial;
- Solution MgCl2 50 mM – 1250 µL x 1 vial.
«ALZYME HSTaq» DNA polymerase, 10х buffer without MgCl2 (2500 units).
- ALZYME HSTaq (5 units/µL) – 500 µL x 1 vial;
- Buffer Taq Mg free 10х – 1250 µL x 5 vials;
- Solution MgCl2 50 mM – 1250 µL x 5 vials.