«ALzyme-HSTaq» DNA polymerase
«ALzyme-HSTaq» DNA polymerase is designed to synthesize a deoxyribonucleic acid (DNA) molecule by complementary copying of DNA template strands during the polymerase chain reaction (PCR). «ALzyme-HSTaq» DNA polymerase is used for:
- Traditional PCR.
- Real-time PCR with TaqMan probes, intercalating dyes.
- Amplification of fragments for TA-cloning.
- Incorporation of modified nucleotides in DNA.
: The enzyme is derived from a recombinant E. coli strain carrying a modified Taq polymerase gene.
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|Reaction buffer for «ALzyme-HSTaq-В» polymerase (c.n. ZHSTaq-B)||1250 µl x 1 vial|
«ALzyme-HSTaq» is a recombinant heat resisting enzyme that catalyzes 5’→3′ single-stranded matrix DNA synthesis in the presence of dNTPs and primers. It has 5’→3′ exonuclease activity, but does not exhibit corrective activity. ALzyme-HSTaq allows efficient amplification of DNA fragments up to 5 bp. Specific mutations make the enzyme resistant to certain inhibitors contained in blood, soil, plant tissue, etc. In addition, ALzyme-HSTaq has deoxynucleotidyl transferase activity, so that a significant portion of the amplified DNA molecules carry protruding deoxyadenosine (dA) residues at the 3′-end.
Hot Start technology. The enzyme is not active under PCR mixture mixing conditions and is activated after the initial denaturation step.
The ALzyme-HSTaq concentration is 5 units/μl. 100 microliters of enzyme (ZHSTaq-100) contains 500 units, 500 µl (ZHSTaq-500) contains 2500 units.
The ALzyme-HSTaq-B reaction buffer provides optimal conditions for ALzyme-HSTaq DNA polymerase.
«ALzyme-HSTaq» DNA polymerase is intended for research purposes.