Description
PURPOSE
«ALPREP Forensic Plus» Extraction kit is designed for nucleic acids extraction from human and animal biological material, and some microorganisms (yeast, gram-negative bacteria) for subsequent polymerase chain reaction analysis. Used at the pre-analytical stage of biological sample analysis in forensic practice for genetic testing, including species/group identification, individual identity verification, genotyping, and establishing biological relationships; as well as for scientific research purposes involving biological samples. The kit includes reagents that ensure the extraction of high-quality DNA and the removal of PCR inhibitors; Proteinase K is included in the kit. «ALPREP BTA» Lysis Buffer is additionally used for nucleic acids extraction from complex matrices such as dental and bone powder (not included in the kit).
ASSAY PRINCIPLE
The method of magnetic sorption is used for DNA and RNA extraction which involves the reversible binding of nucleic acids to the surface of magnetic particles. The sample is treated with a lysis buffer, which leads to the disruption of cell membranes, biopolymer complexes, and the release of nucleic acids. Dissolved nucleic acids bind to the magnetic sorbent particles, while other components of the lysed biological material remain in solution and are removed when the sorbent is pelleted using a magnetic stand. Upon adding the elution buffer to the magnetic sorbent, DNA and RNA are eluted from the sorbent’s surface into the solution. As a result of this procedure, a highly purified nucleic acid preparation is obtained, free from potential inhibitors, ensuring high analytical sensitivity for subsequent analysis.
BIOMATERIAL FOR ANALYSIS
Tissue Material:
- soft tissues, including muscle tissue and dense fibrous tissue;
- soft tissues fixed in formalin and embedded in paraffin;
- nail material (nail plates and subungual content);
- hair fragments with hair follicles.
Dry and wet biological material traces:
- blood (whole blood, plasma, serum);
- seminal fluid (epithelial and sperm fractions after differential lysis);
- epithelium (buccal, skin);
- salivary secretions;
- sputum;
- urine;
- cerebrospinal fluid;
- bronchoalveolar lavage.
Biological material traces collected on carriers (paper, cotton swabs, FTA cards, etc.);
Adhesive materials (adhesive tapes, postage stamps, envelope flaps, cigarette butts, chewing gum, synthetic and natural fabrics, fabrics with complex fibrous structures, soil particles);
Complex matrices (in combination with «ALPREP BTA» lysis buffer):
- bone tissue samples;
- teeth.
ADVANTAGES
- Kit includes Proteinase K which enhances lysis;
- Contains reagents that ensure extraction of high-quality DNA and removal of PCR inhibitors;
- Minimal risk of contamination;
- Certified free from foreign DNA/RNA of various origins (including human DNA/RNA), DNase/RNase in a laboratory accredited to ISO 17025 standards;
- Shelf life – 2 years.
