Description
PURPOSE
«ALPREP Ampli Column» Purification kit is designed for DNA purification from PCR, ligation or restriction reactions using the sorption method on a spin-column membrane. Purified DNA is suitable for subsequent use in PCR, qPCR, restriction, ligation, sample preparation for Sanger and NGS sequencing, as well as in other applications of genetic engineering.
ASSAY PRINCIPLE
The principle of reagent kit involves the reversible binding of nucleic acid to the surface of a silica membrane in a spin column after treating the sample with the kit’s components. The DNA purification protocol includes:
- Processing the sample with buffer solutions;
- Incubation on the membrane column for DNA binding;
- Centrifugation and washing of the bound DNA;
- Elution followed by centrifugation to collect the purified DNA.
ADVANTAGES
- Efficient purification of single- and double-stranded DNA;
- Removal of unbound dNTPs, salts, and other impurities from DNA;
- Purification from fragments up to 100 bp, including primers and their dimers;
- DNA fragment recovery after purification of at least 70%;
- Cost-effectiveness;
- Guaranteed purity and yield of the product;
- Shelf life – 2 years.
