Description
PURPOSE
«ALPREP Forensic Express» Extraction kit is intended for use in forensic practice when carrying out genetic examinations, as well as for research purposes for work with biological samples of humans and animals. It allows to extract DNA from a wide range of forensic and biological samples, suitable for subsequent analysis by polymerase chain reaction. It is recommended to additionally use proteinase K («ALZYME K») for lysis of complex matrices, which is not included in the kit.
ASSAY PRINCIPLE
Assay principle is based on the purification of DNA using an ion exchange resin that precipitates out of solution together with cellular components after centrifugation, while the DNA remains in solution. The resin particles selectively bind polyvalent metal ions, which prevents nucleic acid degradation and facilitates the removal of major PCR inhibitors. This method allows to obtain DNA preparation with minimal probability of contamination and low time consumption, which ensures high productivity of the laboratory.
BIOMATERIAL FOR ANALYSIS
- Soft tissues;
- Nail material (nail plates and subungual contents);
- Hair fragments with hair bulbs;
- Dry and wet biological material traces;
- Blood (whole, plasma, serum);
- Semen (epithelial and sperm fractions after differential lysis);
- Epithelium (buccal, skin);
- Saliva;
- Sputum;
- Urine;
- Cerebrospinal fluid;
- Bronchoalveolar lavage;
- Traces of biomaterial collected on carriers (paper, cotton swabs, FTA cards, etc.);
- Adhesive tapes;
- Postage stamps;
- Envelope seals;
- Cigarette butts;
- Chewing gum;
- Synthetic and natural fabrics;
- Fabrics with complex fibrous structures;
- Soil particles.
ADVANTAGES
- High speed of DNA extraction and purification;
- Contains reagents to ensure removal of PCR inhibitors;
- Minimal risk of contamination;
- Certified free from foreign DNA/RNA of various origins (including human DNA/RNA), DNase/RNase in a laboratory accredited to ISO 17025 standards.
