Description
PURPOSE
«ALZYME SYBR Mix» is a 2X mix of «ALZYME Elaine HSTaq» DNA polymerase, SYBR Green I, dNTPs and all components required for PCR (except DNA matrix and primers). The mix is optimized for efficient and reproducible PCR. The use of «ALZYME SYBR Mix» significantly reduces reaction preparation time and the likelihood of error. Using the «ALZYME SYBR Mix» variant with uracil-DNA glycosylase reduces the likelihood of contamination with PCR products.
Applications:
- real-time PCR with SYBR Green I;
- amplification of DNA fragments for TA cloning;
- direct amplification of DNA from whole blood and plasma.
The «Hot Start» technology ensures that the enzyme is not active under the mixing conditions of the PCR mixture and is activated after the initial denaturation step.