PCR master mix «ALZYME Mix»

Related products

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  ALZYME

  Real-Time PCR master mix «ALZYME SYBR Mix»

  Ready-to-use 2X mix for PCR reactions with SYBR Green I dye.

  Ready-to-use mix for PCR with SYBR Green I.

  • DNA polymerase resistant to PCR inhibitors.
  • Increased stability and specificity.
  • Protection against contamination (ALZYME SYBR Mix variant with UDG).
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  ALZYME

  «ALZYME RT» Reverse Transcriptase

  Recombinant high-fidelity FeVL reverse transcriptase for cDNA synthesis.

  Recombinant high-fidelity FeVL revertase for cDNA synthesis.

  • Synthesis of cDNA fragments up to 12000 bp.
  • Increased thermostability. The enzyme is active at 55 °C.
  • High accuracy and processivity compared to MMLV revertase.
  • The enzyme is devoid of RNase H activity.
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  ALZYME

  «ALZYME Taq» DNA polymerase

  Taq-poly­merase.

  A thermostable DNA polymerase.

  • 5′ → 3′ exonuclease activity.
  • Does not exhibit proofreading activity.
  • Allows efficient amplification of DNA fragments up to 5000 bp.
  • Amplification of fragments for TA cloning.
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  ALZYME

  «ALZYME K» Proteinase

  Purification of enzyme mixtures and cell lysates from unwanted proteins.

  Used for purification of enzyme mixtures and cell lysates from proteins during RNA/DNA isolation.

  • Effectively lyses matrices during nucleic acid isolation.
  • Stable in urea, SDS, EDTA.
  • Inactivates RNases and DNases.
  • It retains activity in the pH range of 6.5-9.5 & at high temperatures (optimally 50-65 °C), functions in the presence of chaotropic salts and detergents.
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  ALZYME

  «ALZYME Elaine Taq» DNA polymerase

  Thermostable Taq-polymerase.

  Thermostable DNA polymerase for direct PCR.

  • 5′ → 3′ exonuclease activity.
  • Resistance to PCR inhibitors.
  • Increased amplification rate.
  • Amplification of fragments for TA cloning.
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  ALZYME

  PCR master mixes «ALZYME Blue Mix», «ALZYME Orange Mix», «ALZYME Green Mix», «ALZYME Red Mix»

  Ready-to-use 2x mixes for PCR reactions with a dye for gel electrophoresis.

  Ready-to-use mixes for PCR with dye for subsequent gel electrophoresis.

  • Direct loading of PCR products into the gel.
  • DNA polymerase resistant to PCR inhibitors.
  • Increased stability and specificity.
  • Protection against contamination (species with uracil-DNA glycosylase).
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  ALZYME

  «ALZYME Bst» DNA polymerase

  Polymerase for loop-mediated isothermal amplification (LAMP).

  DNA polymerase for loop isothermal amplification reaction (LAMP) with displacement activity.

  • 5′ → 3′ polymerase activity.
  • Devoid of 5′ → 3′ & 3′ → 5′ exonuclease activity.
  • Suitable for isothermal amplification reaction.
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  ALZYME

  «ALZYME HSPfu» DNA polymerase

  Highly processive thermostable Hot-start Pfu-polymerase.

  A highly processive thermostable hot-start DNA polymerase.

  • «Hot Start» technology avoids nonspecific amplification and enables room temperature reaction setup.
  • 3’→5′ proofreading activity.
  • Devoid of 5’→3′ exonuclease activity.
  • Allows efficient amplification of DNA fragments up to 20000 bp.
  • Amplification of GC-rich sequences.
  • Resistance to PCR inhibitors.
• 

  ALZYME

  «ALZYME Elaine HSTaq» DNA polymerase

  Thermostable Hot-start Taq-polymerase.

  Thermostable hot-start DNA polymerase for direct PCR.

  • «Hot Start» technology avoids nonspecific amplification and enables room temperature reaction setup.
  • 5′ → 3′ exonuclease activity.
  • Resistance to PCR inhibitors.
  • Increased amplification rate.
  • Amplification of fragments for TA cloning.
• 

  ALZYME

  «ALZYME Pfu» DNA polymerase

  Highly processive thermostable DNA polymerase.

  A highly processive thermostable DNA polymerase.

  • 3′ → 5′ proofreading activity.
  • Devoid of 5′ → 3′ exonuclease activity.
  • Allows efficient amplification of DNA fragments up to 20000 bp.
  • Amplification of GC-rich sequences.
  • Resistance to PCR inhibitors.
• 

  ALZYME

  «ALZYME HSTaq» DNA polymerase

  Hot-start Taq-poly­merase.

  A thermostable hot start DNA polymerase.

  • «Hot Start» technology avoids nonspecific amplification and enables room temperature reaction setup.
  • 5′ → 3′ exonuclease activity.
  • Does not exhibit proofreading activity.
  • Allows efficient amplification of DNA fragments up to 5000 bp.
  • Amplification of fragments for TA cloning.