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«ALZYME HSPfu» DNA polymerase

Highly processive thermostable Hot-start Pfu-polymerase.

A highly processive thermostable hot-start DNA polymerase.

  • «Hot Start» technology avoids nonspecific amplification and enables room temperature reaction setup.
  • 3’→5′ proofreading activity.
  • Devoid of 5’→3′ exonuclease activity.
  • Allows efficient amplification of DNA fragments up to 20000 bp.
  • Amplification of GC-rich sequences.
  • Resistance to PCR inhibitors.
Application field: Forensics, Veterinary, Food safety, Science, Phytosanitary control SKU: ZH-047-200UN | ZH-047-500UN | ZH-047-1KU Category:

Description

PURPOSE

«ALZYME HSPfu» DNA polymerase is used for standard and specialized applications, including:

  • high-fidelity PCR;
  • gene cloning;
  • template generation for sequencing;
  • amplification of GC-rich sequences;
  • long-range PCR (up to 20 kb);
  • direct PCR on blood samples.

«ALZYME HSPfu» DNA polymerase is an alternative to Flash and Phusion DNA polymerases from other manufacturers.
«Hot Start» technology ensures that enzyme is not active under the mixing conditions of the PCR mix and is activated after the initial denaturation step.

CHARACTERISTICS

ALZYME HSPfu is a thermostable chimeric enzyme that catalyzes DNA synthesis, possessing 3′ → 5′ proofreading activity and lacking 5′ → 3′ exonuclease activity. ALZYME HSPfu carries a DNA-affinity domain, which makes the enzyme highly processive and resistant to some inhibitors. ALZYME Pfu B Buffer provides optimal conditions for ALZYME HSPfu DNA polymerase and guarantees increased accuracy and processivity. ALZYME HSPfu concentration is 2 U/μl.

Processivity of ALZYME HSPfu polymerase
Figure 1: Processivity of ALZYME HSPfu polymerase. Electrophoregram of amplification products of 1.7 kb (1), 6 kb (2), and 10 kb (3) fragments of the bacteriophage lambda genome. M – 1 Kb DNA marker.
Resistance of ALZYME HSPfu polymerase to common PCR inhibitors
Figure 2: Resistance of ALZYME HSPfu polymerase to common PCR inhibitors. A 3 kb DNA fragment was amplified in the presence of 2% ethanol (1), 5% ethanol (2), 2% isopropanol (3), 5% isopropanol (4), 80 mM potassium chloride (5), 3 mM sodium citrate (6), 0.01% SDS (7), 6 g/L guanidine hydrochloride (8), 200 mM urea (9). 10 – Control. M – 1 Kb DNA marker.
Stability of ALZYME HSPfu polymerase to human blood components
Figure 3: Stability of ALZYME HSPfu polymerase to human blood components. A 3 kb DNA fragment was amplified in the presence of 0% (1), 1% (2), 3% (3), 5% (4), 7% (5), 10% (6), 15% (7), 20% (8) blood. M – 1 Kb DNA marker.

Release form

Cat. Number Product name Note
ZH-047-200UN «ALZYME HSPfu» DNA polymerase

200 Units:

  • ALZYME HSPfu 2 U/µl, 100 µl - 1 tube;
  • Pfu buffer 10х, 1250 µl - 1 tube.
ZH-047-500UN

500 Units:

  • ALZYME HSPfu 2 U/µl, 250 µl - 1 tube;
  • Pfu buffer 10х, 1575 µl - 2 tubes.
ZH-047-1KU

1000 Units:

  • ALZYME HSPfu 2 U/µl, 500 µl - 1 tube;
  • Pfu buffer 10х, 1250 µl - 5 tubes.
We also recommend
ZPB-047-6.25ML «ALZYME Pfu В» Buffer Pfu buffer 10х, 1250 µl - 5 tubes

Documentation

No available files.

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