«ALZYME Pfu» DNA polymerase

Highly processive thermostable DNA polymerase.

A highly processive thermostable DNA polymerase.

3′ → 5′ proofreading activity.
Devoid of 5′ → 3′ exonuclease activity.
Allows efficient amplification of DNA fragments up to 20000 bp.
Amplification of GC-rich sequences.
Resistance to PCR inhibitors.

Application field: Forensics, Veterinary, Food safety, Science, Phytosanitary control SKU: ZP-047-200UN | ZP-047-500UN | ZP-047-1KU Category: ALZYME

Description

PURPOSE

«ALZYME Pfu» DNA polymerase is used for standard and specialized applications, including:

high-fidelity PCR;
gene cloning;
template generation for sequencing;
amplification of GC-rich sequences;
long-range PCR (up to 20 kb);
direct PCR on blood samples.

«ALZYME Pfu» DNA polymerase is an alternative to Flash and Phusion DNA polymerases from other manufacturers.

CHARACTERISTICS

ALZYME Pfu is a thermostable chimeric enzyme that catalyses DNA synthesis, possessing 3′ → 5′ proofreading activity and lacking 5′ → 3′ exonuclease activity. ALZYME Pfu carries a DNA-affinity domain, which makes the enzyme highly processive and resistant to some inhibitors.

ALZYME Pfu B Reaction Buffer provides optimal conditions for «ALZYME Pfu» DNA polymerase and guarantees increased accuracy and processivity. ALZYME-Pfu concentration is 2 U/μl.

Figure 1: Processivity of ALZYME Pfu polymerase. Electrophoregram of amplification products of 1.7 kb (1), 6 kb (2), and 10 kb (3) fragments of the bacteriophage lambda genome. M – 1 Kb DNA marker.

Figure 2: Resistance of ALZYME Pfu polymerase to common PCR inhibitors. A 3 kb DNA fragment was amplified in the presence of 2% ethanol (1), 5% ethanol (2), 2% isopropanol (3), 5% isopropanol (4), 80 mM potassium chloride (5), 3 mM sodium citrate (6), 0.01% SDS (7), 6 g/L guanidine hydrochloride (8), 200 mM urea (9). 10 – Control. M – 1 Kb DNA marker.

Figure 3: Stability of ALZYME Pfu polymerase to human blood components. A 3 kb DNA fragment was amplified in the presence of 0% (1), 1% (2), 3% (3), 5% (4), 7% (5), 10% (6), 15% (7), 20% (8) blood. M – 1 Kb DNA marker.

Release form

Cat. Number	Product name	Note
ZP-047-200UN	«ALZYME Pfu» DNA polymerase	200 Units: ALZYME Pfu 2 U/µl, 100 µl - 1 tube; Pfu buffer 10х, 1250 µl - 1 tube.
ZP-047-500UN		500 Units: ALZYME Pfu 2 U/µl, 250 µl - 1 tube; Pfu buffer 10х, 1575 µl - 2 tubes.
ZP-047-1KU		1000 Units: ALZYME Pfu 2 U/µl, 500 µl - 1 tube; Pfu buffer 10х, 1250 µl - 5 tubes.
We also recommend
ZTB-047-6.25МL	«ALZYME Pfu В» Buffer	Pfu buffer, 5x1250 µl