PCR master mixes «ALZYME Blue Mix», «ALZYME Orange Mix», «ALZYME Green Mix», «ALZYME Red Mix»

Ready-to-use 2x mixes for PCR reactions with a dye for gel electrophoresis.

Ready-to-use mixes for PCR with dye for subsequent gel electrophoresis.

Direct loading of PCR products into the gel.
DNA polymerase resistant to PCR inhibitors.
Increased stability and specificity.
Protection against contamination (species with uracil-DNA glycosylase).

Description

PURPOSE

«ALZYME Blue Mix», «ALZYME Orange Mix», «ALZYME Green Mix», «ALZYME Red Mix» are 2X mixes of ALZYME Elaine HSTaq DNA polymerase, various dyes, dNTPs and all components required for PCR except DNA matrix and primers. The dyes in the mixture do not inhibit DNA polymerase, facilitate sample application and allow monitoring of DNA migration during electrophoresis. Using these products significantly reduces reaction preparation time and the potential for error. Using «ALZYME Blue Mix», «ALZYME Orange Mix», «ALZYME Green Mix», «ALZYME Red Mix» products with uracil-DNA glycosylase reduces the likelihood of contamination with PCR products.

Applications:

routine PCR;
amplification of DNA fragments for TA cloning;
DNA labeling;
direct amplification of DNA from whole blood and plasma.

The «Hot Start» technology ensures that the enzyme is not active under the mixing conditions of the PCR mixture and is activated after the initial denaturation step.

Release form

For PCR followed by gel electrophoresis analysis

Name	Cat. Number	Number of reactions	Dye	Note
PCR master mix ALZYME Green Mix	ZG-121C-100R	100	Green (Bromothymol blue and Orange G)	Hot Start, chemically inactivated ALZYME Elaine HSTaq
	ZG-121C-1000R	1000	Green (Bromothymol blue and Orange G)	Hot Start, chemically inactivated ALZYME Elaine HSTaq
	ZG-121A-100R	100	Green (Bromothymol blue and Orange G)	Hot Start, Aptamers + ALZYME Elaine HSTaq
	ZG-121A-1000R	1000	Green (Bromothymol blue and Orange G)	Hot Start, Aptamers + ALZYME Elaine HSTaq
PCR master mix ALZYME Blue Mix	ZB-121C-100R	100	Blue (Bromothymol blue)	Hot Start, chemically inactivated ALZYME Elaine HSTaq
	ZB-121C-1000R	1000	Blue (Bromothymol blue)	Hot Start, chemically inactivated ALZYME Elaine HSTaq
	ZB-121A-100R	100	Blue (Bromothymol blue)	Hot Start, Aptamers + ALZYME Elaine HSTaq
	ZB-121A-1000R	1000	Blue (Bromothymol blue)	Hot Start, Aptamers + ALZYME Elaine HSTaq
PCR master mix ALZYME Orange Mix	ZO-121C-100R	100	Orange (Orange G)	Hot Start, chemically inactivated ALZYME Elaine HSTaq
	ZO-121C-1000R	1000	Orange (Orange G)	Hot Start, chemically inactivated ALZYME Elaine HSTaq
	ZO-121A-100R	100	Orange (Orange G)	Hot Start, Aptamers + ALZYME Elaine HSTaq
	ZO-121A-1000R	1000	Orange (Orange G)	Hot Start, Aptamers + ALZYME Elaine HSTaq
PCR master mix ALZYME Red Mix	ZR-121C-100R	100	Red (Cresol Red)	Hot Start, chemically inactivated ALZYME Elaine HSTaq
	ZR-121C-1000R	1000	Red (Cresol Red)	Hot Start, chemically inactivated ALZYME Elaine HSTaq
	ZR-121A-100R	100	Red (Cresol Red)	Hot Start, Aptamers + ALZYME Elaine HSTaq
	ZR-121A-1000R	1000	Red (Cresol Red)	Hot Start, Aptamers + ALZYME Elaine HSTaq

For PCR followed by gel electrophoresis analysis, protection against contamination by PCR products

Name	Cat. Number	Number of reactions	Dye	Note
PCR master mix ALZYME Green Mix	ZG-121CU-100R	100	Green (Bromothymol blue and Orange G)	Hot Start, Chemically Inactivated ALZYME Elaine HSTaq, UDG
	ZG-121CU-1000R	1000	Green (Bromothymol blue and Orange G)	Hot Start, Chemically Inactivated ALZYME Elaine HSTaq, UDG
	ZG-121AU-100R	100	Green (Bromothymol blue and Orange G)	Hot Start, Aptamers + ALZYME Elaine HSTaq, UDG
	ZG-121AU-1000R	1000	Green (Bromothymol blue and Orange G)	Hot Start, Aptamers + ALZYME Elaine HSTaq, UDG
PCR master mix ALZYME Blue Mix	ZB-121CU-100R	100	Blue (Bromothymol blue)	Hot Start, chemically inactivated ALZYME Elaine HSTaq, UDG
	ZB-121CU-1000R	1000	Blue (Bromothymol blue)	Hot Start, chemically inactivated ALZYME Elaine HSTaq, UDG
	ZB-121AU-100R	100	Blue (Bromothymol blue)	Hot Start, Aptamers + ALZYME Elaine HSTaq, UDG
	ZB-121AU-1000R	1000	Blue (Bromothymol blue)	Hot Start, Aptamers + ALZYME Elaine HSTaq, UDG
PCR master mix ALZYME Orange Mix	ZO-121CU-100R	100	Orange (Orange G)	Hot Start, chemically inactivated ALZYME Elaine HSTaq, UDG
	ZO-121CU-1000R	1000	Orange (Orange G)	Hot Start, chemically inactivated ALZYME Elaine HSTaq, UDG
	ZO-121AU-100R	100	Orange (Orange G)	Hot Start, Aptamers + ALZYME Elaine HSTaq, UDG
	ZO-121AU-1000R	1000	Orange (Orange G)	Hot Start, Aptamers + ALZYME Elaine HSTaq, UDG
PCR master mix ALZYME Red Mix	ZR-121CU-100R	100	Red (Cresol Red)	Hot Start, chemically inactivated ALZYME Elaine HSTaq, UDG
	ZR-121CU-1000R	1000	Red (Cresol Red)	Hot Start, chemically inactivated ALZYME Elaine HSTaq, UDG
	ZR-121AU-100R	100	Red (Cresol Red)	Hot Start, Aptamers + ALZYME Elaine HSTaq, UDG
	ZR-121AU-1000R	1000	Red (Cresol Red)	Hot Start, Aptamers + ALZYME Elaine HSTaq, UDG