Набор реагентов «ALSENSE Erwinia amylovora»
Набор реагентов «ALSENSE Erwinia amylovora»
Набор реагентов «ALSENSE Erwinia amylovora»
Набор реагентов «ALSENSE Erwinia amylovora»
Набор реагентов «ALSENSE Erwinia amylovora»
Набор реагентов «ALSENSE Erwinia amylovora»
Набор реагентов «ALSENSE Erwinia amylovora»
Набор реагентов «ALSENSE Erwinia amylovora»

«ALSENSE Erwinia amylovora» Reagent kit

Erwinia amylovora De­tec­tion.

Designed for DNA detection of the causative agent of fruit bacterial blight.

  • High sensitivity and species specificity.
  • Fast assay performance. Ready reaction mix in one tube.
Application field: Phytosanitary control SKU: SEA-075-24 | SEA-075-96 Category:

Description

PATHOGEN INFORMATION

Erwinia amylovora is the causative agent of bacterial blight, the most dangerous necrotic disease of plants of Rosaceae family. The rapid spread of bacteria through host tissues can lead to the death of entire trees in a single growing season. Infection of flowers by Erwinia amylovora can significantly reduce yield and marketability. The phytopathogen is a quarantine organism for countries of the Eurasian Economic Union and countries belonging to the European and Mediterranean Plant Protection Organisation (EPPO A2 list). The economically most important hosts for Erwinia amylovora are Pyrus spp., Malus spp., Cydonia spp., Eriobotrya japonica, Cotoneaster spp., Crataegus spp., Pyracantha spp. & Sorbus spp.

ASSAY PRINCIPLE

«ALSENSE Erwinia amylovora» Reagent kit has been developed based on recommendations of the European and Mediterranean Plant Protection Organization (EPPO). The reagent kit is a multiplex, real-time PCR-based assay. The target for amplification is the ams-operone region of Erwinia amylovora. The internal control (IC) is designed based on the 18S gene sequence of apple and pear ribosomal RNA. The TaqMan® probe to a region of the Erwinia amylovora genome is labeled with a FAM fluorophore dye on the 5′-end. The IC-specific probe is labelled with a HEX fluorophore, allowing the user to validate the DNA extraction step from plants.

SAMPLING RECOMMENDATIONS

«ALSENSE Erwinia amylovora» is validated for the simultaneous detection of Erwinia amylovora & plant DNA by real-time PCR. It is recommended to use samples of flowers, unwooded shoots, leaves and fruits (preferably with symptoms of necrosis and/or exudate) for DNA extraction. Fresh plant tissue samples should be used optimally; however, their storage at +4 to +8 °C for not more than 1 week is acceptable.

Release form

Cat. Number Product name Note
SEA-075-24 ALSENSE Erwinia amylovora

Kit for 24 samples

  • 2x PCR mastermix ERW (1 tube);
  • Positive control ERW (1 tube);
  • Negative control (1 tube).
SEA-075-96

Kit for 96 samples

  • 2x PCR mastermix ERW (4 tubes);
  • Positive control ERW (1 tube);
  • Negative control (1 tube).
We also recommend
PP-100M-50 ALPREP Plant-M Manual Nucleic Acid Extraction Kit for 50 samples
PP-100M-100 Manual Nucleic Acid Extraction Kit for 100 samples
PP-100M-48 ALPREP Plant-M Auto Automated Nucleic Acid Extraction Kit for 48 samples

Documentation

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