Description
PURPOSE
«ALSENSE VetPathogen» kits provide reliable solutions for rapid and accurate detection of bacterial, viral and parasitic agents in animals by real-time PCR. The kits are suitable for use in veterinary and diagnostic laboratories and are released in the following formats:
- Single-target panels for detection of bacterial, viral and parasitic pathogens;
- Combined (multiplex) panels for simultaneous detection of several pathogens;
- Complex panels for screening reproductive, respiratory and gastrointestinal systems of cattle.
Fields of application of «ALSENSE VetPathogen» kits include:
- Laboratory diagnosis of infections in farm animals;
- Epidemiological monitoring and veterinary sanitary surveillance at the herd level;
- Verification/confirmation of diseases in veterinary and research laboratories.
ASSAY PRINCIPLE
The kits are based on real-time polymerase chain reaction (qPCR) using TaqMan probes. The workflow consists of nucleic acid extraction from the specimen followed by real-time amplification of target DNA (or RNA). PCR efficiency monitoring and detection of inhibitors are provided by amplification of a cattle DNA (mRNA) fragment in parallel with the target pathogen DNA (or RNA) fragment.
LIST OF PRODUCTS BY PATOGEN
- ALSENSE VetPathogen Pasteurella
multocida – for detection of Pasteurella
multocida DNA. - ALSENSE VetPathogen Pasteurella
multocida A/D – for differential detection
of Pasteurella multocida group A DNA
and Pasteurella multocida group D DNA. - ALSENSE VetPathogen Mannheimia haemolytica
– for detection of Mannheimia haemolytica DNA. - ALSENSE VetPathogen Mycoplasma
bovis – for detection of Mycoplasma bovis DNA. - ALSENSE VetPathogen Mycoplasma
bovigenitalium – for detection of Mycoplasma
bovigenitalium DNA. - ALSENSE VetPathogen Mycobacterium
spp. – for detection of Mycobacterium spp. DNA. - ALSENSE VetPathogen Mycobacterium
tuberculosis complex – for detection of Mycobacterium
tuberculosis complex DNA (M. tuberculosis, M. bovis,
M. bovis BCG, M. africanum,
M. caprae, M. canettii,
M. microti, M. pinnipediae). - ALSENSE VetPathogen Toxoplasma
gondii – for detection of Toxoplasma gondii DNA. - ALSENSE VetPathogen Francisella tularensis
– for detection of Francisella tularensis DNA. - ALSENSE VetPathogen
CAEV – for detection of caprine arthritis-encephalitis virus (CAEV) DNA. - ALSENSE VetPathogen
LSDV – for detection of the causative agent of cattle nodular dermatitis
(LSDV) DNA. - ALSENSE VetPathogen Histophilus somni
– for detection of Histophilus somni DNA. - ALSENSE VetPathogen Streptococcus
uberis – for detection of Streptococcus
uberis DNA. - ALSENSE VetPathogen Streptococcus
agalactiae – for detection of Streptococcus agalactiae DNA. - ALSENSE VetPathogen Streptococcus
dysgalactiae – for detection of Streptococcus
dysgalactiae DNA. - ALSENSE VetPathogen Trueperella pyogenes – for detection
of Trueperella pyogenes DNA. - ALSENSE VetPathogen Streptococcus
complex – for detection of Trueperella
pyogenes, Streptococcus dysgalactiae,
Streptococcus agalactiae, Streptococcus uberis
DNA. - ALSENSE VetPathogen Staphylococcus
complex – for detection of Staphylococcus aureus DNA and the
methicillin-resistance gene (mecA). - ALSENSE VetPathogen Brucella
spp. – for detection of Brucella spp. DNA. - ALSENSE VetPathogen E.
coli – for detection of E. coli DNA. - ALSENSE VetPathogen E.
coli complex – for detection of genes encoding E. coli
virulence factors F5/F6/F41/eae/sta/stb/LT. - ALSENSE VetPathogen Bovine
herpesvirus 1 (BHV-1) – for detection of the agent of infectious
bovine rhinotracheitis (BHV-1) DNA. - ALSENSE VetPathogen Bovine
herpesvirus 4 (BHV-4) – for detection of bovine herpesvirus
type 4 (BHV-4) DNA. - ALSENSE VetPathogen Bovine
herpesvirus 1/4 (BHV-1/BHV-4) – for detection of bovine herpesvirus
types 1 and 4 DNA. - ALSENSE VetPathogen
BPIV-3 – for detection of parainfluenza virus 3 (BPIV-3) RNA of cattle. - ALSENSE VetPathogen Bovine
viral diarrhea virus – for detection of bovine viral diarrhea virus
(BVDV) RNA. - ALSENSE VetPathogen Klebsiella
pneumoniae – for detection of Klebsiella pneumoniae DNA. - ALSENSE VetPathogen Pseudomonas
aeruginosa – for detection of Pseudomonas aeruginosa DNA. - ALSENSE VetPathogen Listeria
monocytogenes – for detection of Listeria monocytogenes DNA. - ALSENSE VetPathogen Listeria
ivanovii – for detection of Listeria ivanovii DNA. - ALSENSE VetPathogen Listeria
complex – for detection of Listeria monocytogenes and Listeria
ivanovii DNA. - ALSENSE VetPathogen
WNV – for detection of West Nile virus RNA. - ALSENSE VetPathogen Salmonella
spp. – for detection of Salmonella spp. DNA. - ALSENSE VetPathogen Salmonella
complex – for detection of Salmonella spp., S. typhimurium and S.
enteritidis DNA. - ALSENSE VetPathogen Chlamydophila
spp. – for detection of Chlamydophila spp. DNA. - ALSENSE VetPathogen
Bovine respiratory syncytial virus – for detection of bovine respiratory
syncytial virus (BRSV) RNA. - ALSENSE VetPathogen
Bovine coronavirus – for detection of bovine coronavirus RNA. - ALSENSE VetPathogen Cryptosporidium
spp. – for detection of Cryptosporidium spp. DNA. - ALSENSE VetPathogen Yersinia
enterocolitica – for detection of Yersinia enterocolitica
DNA. - ALSENSE VetPathogen Rotavirus
A – for detection of Rotavirus group A RNA. - ALSENSE VetPathogen Rotavirus
C – for detection of Rotavirus group C RNA. - ALSENSE VetPathogen Rotavirus
complex – for detection of Rotavirus group A and group C RNA. - ALSENSE VetPathogen Campylobacter
fetus – for detection of Campylobacter fetus DNA. - ALSENSE VetPathogen Thermophilic
campylobacter – for detection of thermophilic Campylobacter DNA. - ALSENSE VetPathogen Campylobacter
jejuni – for detection of Campylobacter
jejuni DNA. - ALSENSE VetPathogen Campylobacter
complex 1 – for detection of thermophilic Campylobacter and Campylobacter
fetus DNA. - ALSENSE VetPathogen Campylobacter
complex 2 – for detection of thermophilic Campylobacter,
Campylobacter fetus and Campylobacter jejuni
DNA. - ALSENSE VetPathogen Campylobacter
spp. – for detection of Campylobacter spp. DNA. - ALSENSE VetPathogen Ureaplasma diversum
– for detection of Ureaplasma diversum DNA. - ALSENSE VetPathogen Pathogenic
Leptospira – for detection of Pathogenic Leptospira DNA. - ALSENSE VetPathogen Neospora caninum – for detection of Neospora caninum DNA.
- ALSENSE VetPathogen Tritrichomonas foetus
– for detection of Tritrichomonas foetus DNA. - ALSENSE VetPathogen Coxiella
burnetii – for detection of Coxiella burnetii DNA. - ALSENSE VetPathogen Anaplasma marginale
– for detection of Anaplasma marginale DNA. - ALSENSE VetPathogen Anaplasma complex – for detection of Anaplasma marginale
and Anaplasma phagocytophilum
DNA. - ALSENSE VetPathogen
CCHFV – for detection of Congo-Crimean hemorrhagic fever virus RNA. - ALSENSE VetPathogen
BTV – for detection of bluetongue virus (BTV) RNA. - ALSENSE VetPathogen Clostridium
complex 1 – for detection of Clostridium difficile, Clostridium
perfringens DNA and the Clostridium perfringens gene encoding
enterotoxin. - ALSENSE VetPathogen Clostridium
complex 2 – for detection of Clostridium sordellii,
Clostridium septicum, Clostridium chauvoei DNA. - ALSENSE VetPathogen Clostridium
novyi complex – for detection of Clostridium
novyi toxin genes encoding alpha, beta and
gamma toxins. - ALSENSE VetPathogen Mycoplasma
complex 1 – for detection of Mycoplasma bovis
and Mycoplasma bovigenitalium DNA. - ALSENSE VetPathogen Mycoplasma
complex 2 – for detection of Mycoplasma spp. and Ureaplasma diversum
DNA. - ALSENSE VetPathogen
Trio – for detection of Salmonella spp., Listeria monocytogenes
and Chlamydophila spp. DNA. - ALSENSE VetPathogen Eimeria
complex – for detection of Eimeria bovis,
Eimeria zuernii, Eimeria cylindrica and Eimeria
auburnensis DNA. - ALSENSE VetPathogen Corynebacterium
bovis – for detection of Corynebacterium
bovis DNA. - ALSENSE VetPathogen Corynebacterium
pseudotuberculosis – for detection of Corynebacterium
pseudotuberculosis DNA. - ALSENSE VetPathogen
BLV – for detection of bovine leukemia virus (BLV) DNA. - ALSENSE VetPathogen Moraxella
bovis – for detection of Moraxella bovis DNA. - ALSENSE VetPathogen Clostridium
difficile toxin complex – for detection of Clostridium difficile
genes encoding toxin A (tcdA), toxin B (tcdB) and binary toxin (CDT). - ALSENSE VetPathogen Clostridium
perfringens toxin complex – for detection of Clostridium
perfringens toxin genes (CPA, CPB, ETX, ITX). - ALSENSE VetPathogen Chlamydia
complex – for detection of Chlamydia pecorum,
Chlamydia psittaci and Chlamydia
abortus DNA. - ALSENSE VetPathogen
Reproductive complex mini – for detection of pathogens of the cattle
reproductive system (minimal profile). - ALSENSE VetPathogen
Reproductive complex standard – for detection of pathogens of the cattle
reproductive system (standard profile). - ALSENSE VetPathogen
Reproductive complex max – for detection of pathogens of the cattle
reproductive system (extended profile). - ALSENSE VetPathogen
Respiratory complex standard – for detection of respiratory pathogens of
cattle (standard profile). - ALSENSE VetPathogen
Respiratory complex max – for detection of respiratory pathogens of
cattle (extended profile). - ALSENSE VetPathogen
Gastrointestinal complex mini – for detection of gastrointestinal
pathogens of cattle (minimal profile). - ALSENSE VetPathogen
Gastrointestinal complex standard – for detection of gastrointestinal
pathogens of cattle (standard profile). - ALSENSE VetPathogen Klebsiella
spp. – for detection of Klebsiella spp. DNA. - ALSENSE VetPathogen Chlamydia
abortus – for detection of Chlamydia abortus DNA. - ALSENSE VetPathogen Chlamydia
pecorum – for detection of Chlamydia pecorum DNA. - ALSENSE VetPathogen
ASFV – for detection of African swine fever virus (ASFV) DNA. - ALSENSE VetPathogen Suid herpesvirus 1 – for detection of
Aujeszky’s disease virus (Suid herpesvirus
1) DNA. - ALSENSE VetPathogen Borrelia
spp. – for detection of Borrelia spp. DNA. - ALSENSE VetPathogen Pestis
suum – for detection of Pestis suum RNA. - ALSENSE VetPathogen Parvovirus
– for detection of porcine parvovirus DNA. - ALSENSE VetPathogen Influenza
A – for detection of Influenza A virus RNA. - ALSENSE VetPathogen Arterivirus spp. – for detection of arteriviruses (agents of swine
reproductive and
respiratory syndrome) RNA. - ALSENSE VetPathogen Swine
Influenza – for detection of Influenza A subtypes H1 and H3. - ALSENSE VetPathogen Bacillus
anthracis – for detection of Bacillus anthracis DNA
(anthrax). - ALSENSE VetPathogen Porcine
circovirus type 2 – for detection of porcine circovirus type 2
(PCV2) DNA. - ALSENSE VetPathogen Aphtovirus spp. – for detection of Aphthovirus RNA
(foot-and-mouth
disease virus).















