Description
PURPOSE
«ALSENSE VetPathogen» kits provide reliable solutions for rapid and accurate detection of bacterial, viral and parasitic agents in animals by real-time PCR. The kits are suitable for use in veterinary and diagnostic laboratories and are released in the following formats:
- Single-target panels for detection of bacterial, viral and parasitic pathogens;
- Combined (multiplex) panels for simultaneous detection of several pathogens;
- Complex panels for screening reproductive, respiratory and gastrointestinal systems of cattle.
Fields of application of «ALSENSE VetPathogen» kits include:
- Laboratory diagnosis of infections in farm animals;
- Epidemiological monitoring and veterinary sanitary surveillance at the herd level;
- Verification/confirmation of diseases in veterinary and research laboratories.
ASSAY PRINCIPLE
The kits are based on real-time polymerase chain reaction (qPCR) using TaqMan probes. The workflow consists of nucleic acid extraction from the specimen followed by real-time amplification of target DNA (or RNA). PCR efficiency monitoring and detection of inhibitors are provided by amplification of a cattle DNA (mRNA) fragment in parallel with the target pathogen DNA (or RNA) fragment.
LIST OF PRODUCTS BY PATOGEN
- ALSENSE VetPathogen Pasteurella multocida – for detection of Pasteurella multocida DNA.
- ALSENSE VetPathogen Mannheimia haemolytica – for detection of Mannheimia haemolytica DNA.
- ALSENSE VetPathogen Mycoplasma bovis – for detection of Mycoplasma bovis DNA.
- ALSENSE VetPathogen Mycoplasma bovigenitalium – for detection of Mycoplasma bovigenitalium DNA.
- ALSENSE VetPathogen Toxoplasma gondii – for detection of Toxoplasma gondii DNA.
- ALSENSE VetPathogen Histophilus somni – for detection of Histophilus somni DNA.
- ALSENSE VetPathogen Streptococcus uberis – for detection of Streptococcus uberis DNA.
- ALSENSE VetPathogen Streptococcus agalactiae – for detection of Streptococcus agalactiae DNA.
- ALSENSE VetPathogen Streptococcus dysgalactiae – for detection of Streptococcus dysgalactiae DNA.
- ALSENSE VetPathogen Trueperella pyogenes – for detection of Trueperella pyogenes DNA.
- ALSENSE VetPathogen Streptococcus complex – for detection of Trueperella pyogenes, Streptococcus dysgalactiae, Streptococcus agalactiae, Streptococcus uberis DNA.
- ALSENSE VetPathogen Staphylococcus complex – for detection of Staphylococcus aureus DNA and the methicillin-resistance gene (mecA).
- ALSENSE VetPathogen Bovine herpesvirus 1 (BHV-1) – for detection of the agent of infectious bovine rhinotracheitis (BHV-1) DNA.
- ALSENSE VetPathogen Bovine herpesvirus 4 (BHV-4) – for detection of bovine herpesvirus type 4 (BHV-4) DNA.
- ALSENSE VetPathogen Bovine herpesvirus 1/4 (BHV-1/BHV-4) – for detection of bovine herpesvirus types 1 and 4 DNA.
- ALSENSE VetPathogen BPIV-3 – for detection of parainfluenza virus 3 (BPIV-3) RNA of cattle.
- ALSENSE VetPathogen Bovine viral diarrhea virus – for detection of bovine viral diarrhea virus (BVDV) RNA.
- ALSENSE VetPathogen Klebsiella pneumoniae – for detection of Klebsiella pneumoniae DNA.
- ALSENSE VetPathogen Pseudomonas aeruginosa – for detection of Pseudomonas aeruginosa DNA.
- ALSENSE VetPathogen Listeria monocytogenes – for detection of Listeria monocytogenes DNA.
- ALSENSE VetPathogen Salmonella spp. – for detection of Salmonella spp. DNA.
- ALSENSE VetPathogen Chlamydophila spp. – for detection of Chlamydophila spp. DNA.
- ALSENSE VetPathogen Bovine respiratory syncytial virus – for detection of bovine respiratory syncytial virus (BRSV) RNA.
- ALSENSE VetPathogen Bovine coronavirus – for detection of bovine coronavirus RNA.
- ALSENSE VetPathogen Cryptosporidium spp. – for detection of Cryptosporidium spp. DNA.
- ALSENSE VetPathogen Yersinia enterocolitica – for detection of Yersinia enterocolitica DNA.
- ALSENSE VetPathogen Rotavirus A – for detection of Rotavirus group A RNA.
- ALSENSE VetPathogen Campylobacter fetus – for detection of Campylobacter fetus DNA.
- ALSENSE VetPathogen Thermophilic campylobacter – for detection of thermophilic Campylobacter DNA.
- ALSENSE VetPathogen Campylobacter jejuni – for detection of Campylobacter jejuni DNA.
- ALSENSE VetPathogen Campylobacter complex 1 – for detection of thermophilic Campylobacter and Campylobacter fetus DNA.
- ALSENSE VetPathogen Ureaplasma diversum – for detection of Ureaplasma diversum DNA.
- ALSENSE VetPathogen Pathogenic Leptospira – for detection of Pathogenic Leptospira DNA.
- ALSENSE VetPathogen Neospora caninum – for detection of Neospora caninum DNA.
- ALSENSE VetPathogen Tritrichomonas foetus – for detection of Tritrichomonas foetus DNA.
- ALSENSE VetPathogen Coxiella burnetii – for detection of Coxiella burnetii DNA.
- ALSENSE VetPathogen Clostridium complex 1 – for detection of Clostridium difficile, Clostridium perfringens DNA and the Clostridium perfringens gene encoding enterotoxin.
- ALSENSE VetPathogen Mycoplasma complex 1 – for detection of Mycoplasma bovis and Mycoplasma bovigenitalium DNA.
- ALSENSE VetPathogen Trio – for detection of Salmonella spp., Listeria monocytogenes and Chlamydophila spp. DNA.
- ALSENSE VetPathogen Moraxella bovis – for detection of Moraxella bovis DNA.
- ALSENSE VetPathogen Reproductive complex mini – for detection of pathogens of the cattle reproductive system (minimal profile).
- ALSENSE VetPathogen Reproductive complex standard – for detection of pathogens of the cattle reproductive system (standard profile).
- ALSENSE VetPathogen Reproductive complex max – for detection of pathogens of the cattle reproductive system (extended profile).
- ALSENSE VetPathogen Respiratory complex standard – for detection of respiratory pathogens of cattle (standard profile).
- ALSENSE VetPathogen Respiratory complex max – for detection of respiratory pathogens of cattle (extended profile).
- ALSENSE VetPathogen Gastrointestinal complex mini – for detection of gastrointestinal pathogens of cattle (minimal profile).
- ALSENSE VetPathogen Gastrointestinal complex standard – for detection of gastrointestinal pathogens of cattle (standard profile).
- ALSENSE VetPathogen Klebsiella spp. – for detection of Klebsiella spp. DNA.
- ALSENSE VetPathogen Chlamydia abortus – for detection of Chlamydia abortus DNA.
- ALSENSE VetPathogen Chlamydia pecorum – for detection of Chlamydia pecorum DNA.

















