Набор реагентов «ALSENSE Pantoea stewartii subsp. stewartii»
Набор реагентов «ALSENSE Pantoea stewartii subsp. stewartii»
Набор реагентов «ALSENSE Pantoea stewartii subsp. stewartii»
Набор реагентов «ALSENSE Pantoea stewartii subsp. stewartii»

«ALSENSE Pantoea stewartii subsp. stewartii» Reagent kit

For the detection of Pantoea stewartii subsp. stewartii by real-time PCR.

Designed for DNA detection of the causative agent of maize bacterial wilt.

  • High sensitivity and species specificity.
  • Fast assay performance. Ready reaction mix in one tube.
Application field: Phytosanitary control SKU: PSS-104-24 | PSS-104-96 Category:

Description

PATHOGEN INFORMATION

Pantoea stewartii subsp. stewartii is an immobile, non-spore-forming gram-negative bacteria causing bacterial wilt of maize. It mainly affects Zea mays, especially Zea mays subsp. saccharata, sown rice, and some cereals. The phytopathogen is a quarantine organism for countries of the Eurasian Economic Union and countries belonging to the European and Mediterranean Plant Protection Organisation (EPPO A2 list).

ASSAY PRINCIPLE

«ALSENSE Pantoea stewartii subsp. stewartii» Reagent kit has been developed based on recommendations of the European and Mediterranean Plant Protection Organization (EPPO). The reagent kit is a multiplex, real-time PCR-based assay. The target for amplification is the cpsD gene region of Pantoea stewartii subsp. stewartii. The internal control (IC) is designed based on the 18S ribosomal RNA gene sequence of host plants. The TaqMan® probe to a region of the Pantoea stewartii subsp. stewartii genome is labeled with a FAM fluorophore dye on the 5′-end. The IC-specific probe is labelled with a HEX fluorophore, allowing the user to validate the DNA extraction step from plants.

SAMPLING RECOMMENDATIONS

«ALSENSE Pantoea stewartii subsp. stewartii» is validated for the simultaneous detection of Pantoea stewartii subsp. stewartii & plant DNA by real-time PCR. Leaves, flowers and seeds with typical disease symptoms are preferably used as test material.

Release form

Cat. Number Product name Note
PSS-104-24 ALSENSE Pantoea stewartii subsp. stewartii

Kit for 24 samples

  • 2x PCR mastermix PSS (1 tube);
  • Positive control PSS (1 tube);
  • Negative control (1 tube).
PSS-104-96

Kit for 96 samples

  • 2x PCR mastermix PSS (4 tubes);
  • Positive control PSS (1 tube);
  • Negative control (1 tube).
We also recommend
PP-100M-50 ALPREP Plant-M Manual Nucleic Acid Extraction Kit for 50 samples
PP-100M-100 Manual Nucleic Acid Extraction Kit for 100 samples
PP-100M-48 ALPREP Plant-M Auto Automated Nucleic Acid Extraction Kit for 48 samples

Documentation

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