Набор реагентов «ALSENSE Phytoplasma mali/pyri»
Набор реагентов «ALSENSE Phytoplasma mali/pyri»
Набор реагентов «ALSENSE Phytoplasma mali/pyri»
Набор реагентов «ALSENSE Phytoplasma mali/pyri»
Набор реагентов «ALSENSE Phytoplasma mali/pyri»
Набор реагентов «ALSENSE Phytoplasma mali/pyri»
Набор реагентов «ALSENSE Phytoplasma mali/pyri»
Набор реагентов «ALSENSE Phytoplasma mali/pyri»

«ALSENSE Phytoplasma mali/pyri» Reagent kit

Phyto­plasma mali/pyri De­tec­tion.

Designed for DNA detection of pear decline phytoplasma and apple proliferation phytoplasma.

  • High sensitivity and species specificity.
  • Fast assay performance. Ready reaction mix in one tube.
Application field: Phytosanitary control SKU: SPMP-076-24 | SPMP-076-96 Category:

Description

PATHOGEN INFORMATION

Apple proliferation phytoplasma (Candidatus Phytoplasma mali) and pear decline phytoplasma (Candidatus Phytoplasma pyri) are the causative agent of serious diseases in apple and pear trees, causing significant economic damage to fruit growing across Europe.
These bacteria belong to the 16SrX taxonomic group and are considered quarantine organism for Eurasian Economic Union countries, and member countries of European and Mediterranean Plant Protection Organization (EPPO A2 list). Pyrus spp., Malus spp., Cydonia spp. are the most economically significant hosts for Candidatus Phytoplasma pyri & Candidatus Phytoplasma mali.

ASSAY PRINCIPLE

«ALSENSE Phytoplasma mali/pyri» Reagent kit has been developed based on recommendations of the European and Mediterranean Plant Protection Organization (EPPO). The reagent kit is a multiplex, real-time PCR-based assay. The target for amplification is the 16S ribosomal RNA gene region of phytoplasmas of the 16SrX group. The internal control (IC) is designed based on the 18S gene sequence of apple and pear ribosomal RNA. The TaqMan® probe to a region of the Candidatus Phytoplasma pyri & Candidatus Phytoplasma mali genomes is labeled with a FAM fluorophore dye on the 5′-end. The IC-specific probe is labelled with a HEX fluorophore, allowing the user to validate the DNA extraction step from plants.

SAMPLING RECOMMENDATIONS

«ALSENSE Phytoplasma mali/pyri» is validated for the simultaneous detection of Candidatus Phytoplasma pyri & Candidatus Phytoplasma mali and plant DNA by real-time PCR. It is recommended to use samples of shoots, petioles and midveins of leaves with disease symptoms for preliminary DNA extraction. Fresh plant tissue samples are optimal, but storage at minus 20 to minus 80°C is acceptable.

Release form

Cat. Number Product name Note
SPMP-076-24 ALSENSE Phytoplasma mali/pyri

Kit for 24 samples

  • 2x PCR mastermix PMPP (1 tube);
  • Positive control PMPP (1 tube);
  • Negative control (1 tube).
SPMP-076-96

Kit for 96 samples

  • 2x PCR mastermix PMPP (4 tubes);
  • Positive control PMPP (1 tube);
  • Negative control (1 tube).
We also recommend
PP-100M-50 ALPREP Plant-M Manual Nucleic Acid Extraction Kit for 50 samples
PP-100M-100 Manual Nucleic Acid Extraction Kit for 100 samples
PP-100M-48 ALPREP Plant-M Auto Automated Nucleic Acid Extraction Kit for 48 samples

Documentation

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