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«ALCHEM MgCl2 50 mM» Solution

For optimization of Mg2+ ion concentration in the PCR Master mix.

For optimization of Mg2+ ion concentration in the PCR Master mix.

Application field: Forensics, Veterinary, Science SKU: CM-109-1 Category:

Description

Description

«ALCHEM MgCl2 50 mM» Solution is used for optimization of Mg2+ ion concentration in the PCR Master mix. A typical final concentration for Mg2+ in PCR is in the range of 1–4 mM, with 0.5 mM titration increments recommended for optimization.

Mg2+ ions:

  • Ensure the polymerase activity;
  • Are necessary for coordinating dNTPs in the enzyme’s active site, which facilitates the transfer of phosphate groups and the formation of phosphodiester bonds in the new DNA strand;
  • Affect the hybridization temperature of primers with DNA;
  • Provide specificity and efficiency of the PCR reaction.

Release form

Cat. Number Product name Note
CM-109-1 ALCHEM MgCl2 50 mM Solution 1 ml bottle

Documentation

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