Description
PURPOSE
«ALMUNO-IgGRBD-SARS-CoV-2» Kit is designed for the determination of IgG to the RBD part of glycoprotein S (spike) of SARS-CoV-2 virus in human serum and plasma, which have viral neutralizing activity due to blocking the binding of the virus to the human cell receptor ACE2. The information about the content of such immunoglobulins allows to evaluate:
- the risk of re-infection;
- suitability of donor plasma for transfusion to patients with severe coronavirus infection;
- vaccination efficacy (assuming vaccination with non-vector vaccines).
The kit is designed for 96 determinations, including staging in triplicate of the negative control and in monoplicate of the positive control. The kit is intended for in vitro use only.
PRINCIPLE OF METHOD
The detection is based on indirect noncompetitive two-step ELISA. At first step samples are incubated in the microtiter plate, precoated with a recombinant SARS-CoV-2 RBD. Immunoglobulins to this protein when present in the sample bind specifically to the antigen immobilized in the wells. Next, IgG-class antibodies are detected with enzyme conjugate (HRP-labelled anti-human IgG antibody). After incubation with the TMB Substrate and stop reagent application, the solution optical density is measured. The measured optical density is directly related to the amount of IgG to SARS-CoV-2 RBD in the sample.
CHARACTERISTICS
- sensitivity: 100%;
- specificity: 100%;
- complete ELISA kit;
- 12 months warranty period.
