Description
PURPOSE
«ALMUNO Antibiotic Streptomycin» Kit is intended for quantitative determination of streptomycin by ELISA in the following matrices:
- milk (raw, pasteurized, sterilized), reconstituted milk powder, reconstituted infant food;
- yoghurt, sour cream, reconstituted whey powder, milk whey, milk-based shakes, ice cream, cottage cheese, butter, cheese;
- condensed milk, honey;
- meat, meat products, eggs, offal (liver, kidneys), tinned meat and meat and vegetable products, fat, lard, including bacon.
PRINCIPLE OF METHOD
The method is based on a competitive colorimetric enzyme-linked immunoassay. During the analysis streptomycin from the sample competes with the antibiotic molecules immobilized on the surface of the microplate wells for binding to antibodies (anti-streptomycin). The resulting complex is detected using HRP-conjugated secondary antibodies. The concentration of streptomycin in the sample is determined from a calibration curve constructed with 6 calibration solutions.
DETECTION LIMITS & MEASURMENT RANGES
| Matrix | Measuring range, µg/kg (µg/L) |
|
Milk (raw, pasteurized, sterilized), reconstituted milk powder, reconstituted infant food |
5 – 375 |
|
Yoghurt, kefir, sour cream, reconstituted whey powder, milk whey, milk-based shakes, ice cream, cottage cheese, butter, cheese |
7,50 – 375 |
| Condensed milk, honey | 20 – 600 |
|
Meat, meat products, eggs, by-products (liver, kidneys), tinned meat and meat andegetable products |
10 – 1200 |
| Fat, lard, including bacon | 10 – 1200 |
BENEFITS
- low quantification limit – 5 µg/L for milk, 7,5 µg/kg for dairy products and 10 µg/kg for meat and meat products;
- a possibility to perform ELISA with more than six strips at the same time;
- software in the form of a template for automatic calculation of results;
- shelf-life when stored properly: 18 months.
