Description
PURPOSE
«ALMUNO Penicillin» Kit is a test system based on a recombinant penicillin-binding protein designed by Algimed Techno.
The kit enables the quantitative determination of penicillin-group antibiotics using the competitive enzyme-linked immunosorbent assay (ELISA) method in the following matrices:
- Milk (raw, pasteurized, sterilized), cream, reconstituted dry milk, dairy desserts, condensed milk, milk ice cream, dairy-based drinks and cocktails (including yogurt-based), milk porridges, reconstituted dry milk porridges, infant formulas and reconstituted dry milk infant formula;
- Butter, milk fat, spread;
- Cheese, cheese products, cheese pastes, and culinary cheese-based products;
- Cottage cheese and cottage cheese products (including cottage cheese bars, granular cottage cheese, cottage cheese mass, cottage cheese paste, cottage cheese creams, and similar products with or without fillers), cottage cheese-based cheese, fermented dairy products (ayran, yogurt, acidophilin, and similar products with or without fillers), sour cream, buttermilk, whey, reconstituted dry whey, whey-based beverages, permeate;
- Meat, meat products, meat-vegetable products, lard, pork fat, animal fats, by-products;
- Poultry meat, poultry products, semi-finished poultry products, canned poultry products, poultry by-products;
- Fish, aquacultural products, including infant food (culinary fish products and semi-finished products, canned and preserved fish, caviar, boiled-frozen fish food products), shrimps;
- Eggs, egg products, and processed egg products (including egg powder, liquid egg mass, and melange);
- Honey, creamed honey;
- Blood serum and plasma.
PRINCIPLE OF METHOD
The method is based on a competitive colorimetric enzyme-linked immunosorbent assay (ELISA). During the analysis, a biotinylated penicillin-binding protein (PBP) is added to the wells of a microplate coated with the antibiotic, along with the sample. If the sample contains a penicillin-group antibiotic, it competes with the coated antibiotic for binding to the PBP, thereby preventing its attachment to the microplate wells. The resulting complexes are detected using a streptavidin-peroxidase conjugate. The mass concentration of the penicillin-group antibiotic, expressed as benzylpenicillin, is determined based on a calibration curve constructed with six penicillin G calibration solutions.
DETECTION LIMITS
| Matrix | Measuring range, µg/kg (µg/L) |
| Milk (raw, pasteurized, sterilized, UHT), cream, reconstituted dry milk, dairy-based drinks and cocktails (including yogurt-based), infant formulas and reconstituted dry milk infant formulas | 0,4-10 |
| Dairy desserts, milk ice cream, milk porridges, reconstituted dry milk porridges, cottage cheese and cottage cheese products, fermented dairy products, sour cream, buttermilk, whey, reconstituted dry whey, whey-based beverages, permeate | 0,8-20 |
| Butter, milk fat, cream-vegetable spread, condensed milk | 0,8-20 |
| Cheese, cheese products and cheese pastes, culinary cheese-based products | 0,8-20 |
| Meat, meat products, meat and meat-vegetable semi-finished products and canned goods, lard, pork fat, animal fats, poultry meat, poultry products, semi-finished poultry products, canned poultry products, fish, processed and unprocessed fish food products, shrimps | 0,8-20 |
| By-products, poultry by-products, and their processed products | 1,6-40 |
| Eggs, egg products, and processed egg products | 0,8-20 |
| Honey, creamed honey | 1,6-40 |
| Blood serum and plasma | 1,6-40 |
SPECIFICITY
| Analyte | Cross-reactivity (%) |
| Benzylpenicillin (Penicillin G) | 100 |
| Ampicillin | 100 |
| Amoxicillin | 22 |
| Dicloxacillin | 63,2 |
| Nafcillin | 10 |
| Penicillin V | 67,0 |
| Oxacillin | 36,7 |
| Ceftiofur | 0,42 |
| Cefazolin | 0,87 |
| Cefquinome | 2,52 |
| Cefotaxime | 0,09 |
KIT COMPOSITION
| Item | Quantity |
| Immunosorbent | 12 strips of 8 wells each |
| Empty vials for calibration solution preparation | 8 pcs |
| Primary Penicillin G standard (1000 ng) | 2 pcs |
| Penicillin G spike reagent (1000 ng) | 1 pc |
| PBP concentrate (50x) | 1x0,3 ml |
| PBP dilution buffer | 1x15 ml |
| Conjugate | 1x16 ml |
| Extraction buffer concentrate (10x) | 1x100 ml |
| Wash buffer concentrate (20x) | 1x30 ml |
| Stop reagent | 1x12 ml |
| TMB substrate | 1x12 ml |
ADVANTAGES
- software as a template for automatic result calculation;
- wide application range: the kit allows the analysis of various products, including meat, fish, dairy products, eggs, serum, and blood plasma;
- simplicity and convenience: intuitive sample preparation methods save time and resources;
- high sensitivity: a limit of quantification from 0.4 µg/kg ensures reliable results even at minimal antibiotic concentrations;
- high specificity: the test demonstrates low cross-reactivity with cephalosporins, ensuring reliable detection of penicillin-group antibiotics;
- shelf-life when stored properly: 18 months.
