Description
PURPOSE
The system for mass spectrometric analysis «ALMASS Bio» is designed for fast and reliable identification of microorganisms by mass spectrometry. Bioinformatic algorithms of the software guarantee accurate species identification of microorganisms by comparing the obtained mass spectra with an extensive database. Identification is based on the protein spectrum unique for each microorganism species: ribosomal proteins are specific for microorganisms. It is realised in the versions «ALMASS Bio 60» and «ALMASS Bio 200».
PRINCIPLE OF METHOD
The principle of the method is based on the preparation of analysed samples, processing with a material (matrix) reducing destructive properties of laser radiation and obtaining mass spectra of protein profiles. The obtained spectrum is compared with a library of known spectra of microorganisms. Each microorganism species has a unique protein spectrum, which allows its precise identification.
TECHNICAL CHARACTERISTICS
| Characterisation | ALMASS Bio 60 | ALMASS Bio 200 |
| Solid-state laser | Up to 60 Hz, continuously adjustable; life over 1010 shots | Up to 200 Hz continuously adjustable; life over 1010 shots |
|
Number of identifications ( * on an 8-hour working day on real samples) |
Over 2,000 * | Up to 3,000 * |
| Ionisation mode | Positive for routine identification tasks |
Positive for routine identification tasks. Negative for protein profiling, glycotyping, m/o differentiation, including for antibiotic sensitivity / resistance tasks |
| Target | Reusable, stainless steel | |
| Identification objects | Bacteria, mycobacteria, fungi, yeast | |
| Database | Over 2,700 species and 7,000 strains with the possibility of database supplementation by the user | |
| Resolution | Over 4,000 at m/z 1619 (Bombesin) | |
| Detection limit | S/N > 10:1 at m/z 445 (Tetracycline, 0.1 mg/ml) | |
| Relative RMS | Not more than 0.02 % at m/z 445 (Tetracycline, 0.1 mg/ml) | |
| Accuracy of masses | Not more than 200 ppm | |
| Mass range | 1 – 300,000 Da | |
| Stability of masses | Less than 200 ppm/8 h | |
| Loading a target | ~ 1 min | |
| Single identification time | ~ 10 sec | |
| Performance | ~ 300 identifications/h (1 target – 96 identifications ~ 20 min) | |
BENEFITS
- solid-state laser ensures durability of the devices – service life is not less than 10 years;
- Reusable steel targets – saving laboratory costs;
- best resolution in its class – more accurate and better identification;
- excellent performance of the devices – over 2000 identifications in 1 working day;
- optimised device design without external sources of noise and heat generation;
- an ergonomic workstation that takes into account any needs that arise during operation;
- Unified software in Russian, intuitive interface – user-friendly for any laboratory;
- standard database and software update during annual maintenance;
- visually recognisable indication of the device status;
- own local database of microorganisms: more than 2700 species and 7000 strains with the possibility of independent supplementation by the user;
- supplementation of the database from Russian collections;
- LIS/LIMS integration;
- registered for IVD in the territory of the Russian Federation.
EFFICIENT WORKPLACE ORGANIZATION
The system is additionally supplied with:
- «ALMASS Bench» for HPLC-MS is designed to accommodate HPLC-MS systems;
- «ALMASS» bench for MALDI-TOF MS (SP-120) is designed for installation of mass spectrometers and their accessories, as well as other medical and laboratory equipment;
- «ALMASS Bio» reagent kits are intended for preparation of samples of microorganisms of 3-4 pathogenicity groups for determination of their species affiliation and for calibration of MALDI-TOF mass spectrometers by typical protein profile of Escherichia coli bacteria.
