Description
PURPOSE
«ALFLOW Prep Fix» is a reagent that ensures the fixation of leukocytes in the sample intended for flow cytometry. It stabilizes the membranes of white blood cells after the lysis procedure and preserves the prepared sample until measurement on a flow cytometer.
PRINCIPLE OF THE PROCEDURE
After incubation with the lysing reagent, which has a targeted effect, the erythrocyte membrane is destroyed into fragments that are smaller than the cells in the target leukocyte populations. The addition of fixative solution stabilizes the leukocyte membranes, which increases the storage time of the prepared sample until the measurement is performed.