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«ALFLOW Count» Fluorescent Microspheres

Direct determination of absolute cell counts in samples intended for flow cytometry analysis.

Designed for single-platform determination of absolute cell counts by flow cytometry.

  • Ready-to-use suspension of fluorescent microspheres.
  • Can be used with flow cytometers that are equipped with a blue laser or blue and red lasers.
Application field: Medicine, Science SKU: FС-032-50 | FС-032-100 | FС-032-200 | FC-108-50 | FC-108-100 | FC-108-200 Category:

Description

PURPOSE

«ALFLOW Count» Fluorescent Microspheres are a suspension of fluorescent polystyrene microbeads that have uniform size and fluorescence intensity. They are designed to determine absolute cell counts by flow cytometry. «ALFLOW Count» Fluorescent Microspheres can be used with flow cytometers equipped with a blue laser (488 nm) or blue and red lasers.

PRINCIPLE OF USE

Fluorescent microspheres are ready to use. No additional preparation is necessary. The principle of test depends upon mixing a known volume and concentration of ALFLOW Count Microspheres with an identical volume of a biological specimen. During the analysis on a flow cytometer, the absolute number (cells/μL) of cells in the sample can be determined by comparing cellular events to fluorescent microspheres events. Since the concentration of fluorescent microspheres is predetermined, the ratio of the number of cells to the number of microspheres can be determined and the absolute number of cells in the sample can be calculated.

Release form

Cat. Number Product name Note
FC-108-50 ALFLOW Count Fluorescent microspheres; 5 ml vial (50 tests)
FC-108-100 Fluorescent microspheres; 10 ml vial (100 tests)
FC-108-200 Fluorescent microspheres; 20 ml vial (200 tests)
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Documentation

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