«ALMIR» reagent kits

MiRNAs are short ribonucleic acid molecules (20–22 nucleotides) that regulate genome expression on post­transcription level. Alteration of miRNA regulatory function is strongly associated with cancer development. Therefore, miRNAs are considered as promising markers for cancer diagnostics as well as targets for anticancer therapy. PCR is the main method of microRNA analysis in laboratory practice due to its reliability and cost-effectiveness. ALMIR reagent kits for PCR analysis of microRNA are designed to determine the concentration of certain small regulatory RNA molecules in a mixture of RNA extracted from biological material (tissues, biological fluids).

These kits may be used in basic research, the development of new methods of clinical diagnosis, the study of gene expression, etc. The kits are intended for in vitro use only.

The kit implements the original technology of two-way priming of reverse transcription and PCR with two micro-RNA-specific primers, which provides highest analysis accuracy. Optimization of the RT-primer structure and detection of amplification using a probe ensures reliable results and higher sensitivity of the assay compared to other technologies. This allows working with low concentration RNA samples.

• exceptional accuracy and specificity;
• calibration curves and quantitative analysis on a set of eight calibrators;
• microRNA quantification even from 1 pkg of total RNA;
• fast two-step protocol lasting less than 3 hours;
• the possibility to develop a panel according to the customer’s needs;
• 12 months warranty period.