«ALSENSE Xylella fastidiosa» Reagent kit
«ALSENSE Xylella fastidiosa» Reagent kit is designed to detect specific Xylella fastidiosa DNA by PCR based on the TaqMan technology in nucleic acid preparations.
Xylella fastidiosa is a gram-negative bacterium that parasitizes xylem vessels. The pathogen affects about 400 plant species from 75 different botanical families, among which the most important are grapes, plum, pear, olive, almond, blackberry and rosehip. The phytopathogen is a quarantine object for the Republic of Belarus, countries of the Eurasian Economic Union and countries belonging to the European and Mediterranean Plant Protection Organisation (EPPO A2 list).
The kit is designed based on the recommendations of the European and Mediterranean Plant Protection Organisation (EPPO) and allows the detection of pathogen DNA by real-time polymerase chain reaction. The target for amplification is the rRNA 16S gene region of Xylella fastidiosa. An internal control (IC) was designed based on the grape rRNA 18S gene sequence. The TaqMan® probe to the Xylella fastidiosa genome region carries a FAM dye tag at the 5'-end. The IC-specific probe is labelled with a HEX fluorophore, allowing the user to validate the DNA extraction from plants.
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The assay principle of «ALSENSE Xylella fastidiosa» Reagent kit is to conduct real-time polymerase chain reaction (real-time PCR) with hybridisation-fluorescence detection on a DNA matrix. The PCR method is based on the repeated doubling of a specific DNA region using DNA polymerase in vitro. As a result, DNA copies are produced in sufficient quantity to reliably determine the result. Only the specific DNA region that meets the specificity conditions set in the structure of primers and fluorescent probe is copied during the reaction, and only if it is present in the sample being tested.
- SXF-104-24
- SXF-104-96
«ALSENSE Xylella fastidiosa» (SXF-104-24, 24 reactions):
- 2x PCR mastermix XF – 1 vial;
- PCS XF – 1 vial;
- NCS – 1 vial.
«ALSENSE Xylella fastidiosa» (SXF-104-96, 96 reactions):
- 2x PCR mastermix XF – 4 vials;
- PCS XF – 1 vial;
- NCS – 1 vial.