«ALSENSE Forensic Quantify» Real-Time PCR Kit for human DNA quantity and sex determination
«ALSENSE Forensic Quantify» Real-Time PCR Kit is based on a real-time polymerase chain reaction with hybridization-fluorescence detection using TaqMan-specific probes.
Reagent kit is intended for use in forensics, as well as PCR studies in biology and medicine.
Reagent kit is designed to determine, estimate the amount of human genomic DNA and sex by real-time polymerase chain reaction in human biological material.
Amplification of multi-copy autosomal fragments of different sizes allows a reliable DNA assessment in the studied sample, and amplification of a multi-copy Y chromosome fragment – sex determination.
The table shows the targets and dyes used:
|Y chromosome DNA||FAM|
DNA concentrations are determined using a calibration line plotted against four points with the following concentrations:
50 ng/µl, 2 ng/µl, 0.08 ng/µl, 0.0032 ng/µl.
«ALSENSE Forensic Quantify» Real-Time PCR Kit (for 100 samples):
- PCR buffer – 1 vial;
- Polymerase – 1 vial;
- Negative control sample – 1 vial;
- Calibrator – 1 vial;
- Calibrator dilution buffer – 1 vial.