Product lines
«ALPREP-Ampli» reagent kit
Item code: Ampli‑1 | Ampli‑2
The purified DNA is suitable for subsequent use in PCR, real time PCR, restriction, ligation, sample preparation for Sanger and NGS sequencing, and other genetic engineering applications.
The kit is intended for research purposes.
Characteristics
- Single- and double-stranded DNA purification from reaction mixtures.
- Effective DNA purification from unbound dNTPs, salts, and other impurities.
- Purification of fragments up to 100 bp (including primers and their dimers).
- Of DNA fragments yield after purification from 70%.
Operation principle
Kit content
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Operation principle
The operation principle of «ALPREP-Ampli» reagent kit (product forms 1, 2) is the reversible binding of nucleic acid molecules on the surface of magnetic sorbent particles after treatment of the used sample with the kit components.
Kit content
- Ampli‑1
- Ampli‑2
«ALPREP-Ampli» reagent kit for DNA purification from reaction mixtures (c.n. Ampli‑1, 50 samples):
- AmpliClean Buffer – 10,5 mL × 1 pc;
- Bind buffer – 18,4 mL × 1 pc;
- Wash buffer – 31,5 mL × 1 pc;
- Elute buffer – 4,2 mL × 1 pc;
- Magnetic sorbent – 1 mL × 1 pc.
«ALPREP-Ampli» reagent kit for DNA purification from reaction mixtures (c.n. Ampli‑2, 100 samples):
- AmpliClean Buffer – 21 mL × 1 pc;
- Bind buffer – 36,8 mL × 1 pc;
- Wash buffer – 63 mL × 1 pc;
- Elute buffer – 8,4 mL × 1 pc;
- Magnetic sorbent – 2 mL × 1 pc.
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