Real-Time PCR kit ALSENSE-SARS-CoV-2-RT-qPCR qualitative identification by RT-PCR method
Real-Time PCR Kit «ALSENSE-SARS-CoV-2-RT-qPCR», Cat.N ALSARS5-96 are designed to detect RNA from coronavirus including «British» (20I/501Y.V1, VOC 202012/01, or «alpha» B.1.1.7), «South African» (20H/501Y.V2, «beta» B.1.351., «omicron» variant B.1 .1.529), as well as «Brazilian» («gamma» P.1) and «Indian» (AY.1 or «delta» B.1.617.2,) strains by a single-step polymerase chain reaction with real-time fluorescence detection.
The kit detects conserved sequences in the E and N genes of coronavirus 2019-nCoV. Currently gene sequences of wild-type, «British», «South African» and «Brazilian» strains of 2019-nCoV coronavirus have been identified by sequencing. The gene sequences determined by the «ALSENSE-SARS-CoV-2-RT-qPCR» reagent kit correspond to the gene sequences of all the above strains.
Therefore, «ALSENSE-SARS-CoV-2-RT-qPCR» reagent kits allows the detection of «British», «South African», «Brazilian» strains of coronavirus 2019-nCoV, but does not allow its differentiation from all other strains of coronavirus 2019-nCoV.
Real-Time PCR kit ALSENSE-SARS-CoV-2-RT-qPCR for the qualitative detection of SARS-CoV‑2 RNA by one step real-time fluorescent reverse transcription PCR technology in nucleic acid (NA) preparations isolated from clinical samples.
Video instruction for using the kit
The working principle is based on a one-step real-time fluorescent reverse transcription PCR technology.
PurposeReal-Time PCR kit ALSENSE-SARS-CoV-2-RT-qPCR is intended to use when 2019-nCoV coronavirus infection is suspected (in case of fever, fatigue, loss of appetite, cough (dry), sore throat, headache or shortness of breath).
Sensitivity- from 100 copies/ml;
- from 5 genomic equivalents in reaction.
The kit detects the following genes: 3 loci of the 2019-nCoV coronavirus ORF1ab gene and 2019-nCoV coronavirus S, E, and N genes
Application in clinical laboratory diagnostics and in research practice for the qualitative determination of RNA of coronavirus 2019-nCoV clinical samples (nasal secret, nasopharyngeal swab, oropharyngeal swab, sputum, endotracheal aspirate, bronchoalveolar rinse, rectal smear, plasma, human blood serum) in specialized laboratories of medical and healthcare institutions. For in vitro studies only.
Kits are configured to process 48/96 clinical samples. Real-Time PCR kit ALSENSE-SARS-CoV-2-RT-qPCR includes all necessary reagents for RT-PCR, including inner control, negative control and positive control.
| Release form | Reagent | Volume | Amount |
| Release form 4, for 48 assays | ALSENSE reaction buffer | 0.75 mL | 1 tube |
| ALSENSE RT-polymerase | 0.06 mL | 1 tube | |
| PSC | 0.1 mL | 1 tube | |
| NSC | 0.5 mL | 1 tube | |
| Release form 5, for 96 assays (auto) | ALSENSE reaction buffer | 1.5 mL | 1 tube |
| ALSENSE RT-polymerase | 0.12 mL | 1 tube | |
| PSC | 0.1 mL | 1 tube | |
| NSC | 0.5 mL | 1 tube | |
| Release form 6, for 96 assays | ALSENSE reaction buffer | 96 x 0.16 mL | 1 tube |
| Plate sealing pad | 1 pcs | 1 package | |
| PSC | 0.1 mL | 1 tube | |
| NSC | 0.5 mL | 1 tube |