ELISA-IgG-SARS-CoV‑2 test kit

Item code: E001‑G

Diagnostic test kit created to detect immunoglobulins G by ELISA method. Kit is configured to analyze 46 unknown samples in duplicates, negative control triplicate, positive control monoplicate. 96 definitions in total.

ELISA-IgG-SARS-CoV‑2 kit has been created for in vitro detection of IgG antibodies to SARS-CoV   2 in serum, plasma and human whole blood.

Immunoglobulin G (IgG) represents about 75% of the human blood plasma antibodies. It is the most common antibody in the blood flow. IgG is primarily involved in the secondary immune response, secreted in the form of monomers that easily penetrate the tissue.

The IgG measurement to SARS-Cov‑2 may help to determine immune response in patients with COVID-19 disease, as well as to estimate the antibodies level in blood plasma in patients recovered.

Kit componentsQuantityStorage
Immunoadsorbent1 pce2 – 8°С
Conjugate12 mL2 – 8°С
Positive Control0,5 mL2 – 8°С
Negative Control1 mL2 – 8°С
Sample Diluent100 mL2 – 8°С
Wash Concentrate(20x)*20 mL2 – 8°С
TMB Substrate *12 mL2 – 8°С
Stop Solution *12 mL2 – 8°С

The working principle is based on indirect non-competitive ELISA analysis. The study and control samples are incubated in the wells coated with a recombinant nucleocapsic protein. Immunoglobulins to this protein when present in the sample bind specifically to the protein immobilized in the wells. Next, Igm-class antibodies are detected with the help of a second antibody in a peroxidase conjugate. After incubation with the substrate and stop solution application, the solution optical density at 450 nm is measured. The measured optical density is directly related to the number of IgМ in the study and control samples.

The study and control samples are incubated in the wells coated with a recombinant