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ELISA antibiotic chloramphenicol test kit
Item code: 013–05BA
Test kit is used for chloramphenicol detection.
Key Benefits
Kit Content
Measuring detection limits
Key Benefits
Description: this test kit is used to determine chloramphenicol (levomycetin) in the following matrices:
- Milk (raw, pasteurized, sterilized)
- Milk powder
- Meat
- Honey
- Cheese
- Butter
- Eggs
- Ready-to-eat meat products
- Cottage cheese
- Yogurt
- Kefir
- Sour cream
- Reconstituted milk whey, milk powder whey
- Reconstituted milk powder infant formula
- Milk-based cocktails
- Salo
- Speck
- Animal fat
- Fish
- Fish products
- Shrimps
- By-products
- Canned meat
Use primary filter 450 nm
Overview:
- Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards).
- All unused wells can be stored with the desiccant sealed in the foilpackage.
- Storage temperature: 2–8 °С.
- Shelf-life when stored properly: 12 months.
Kit Content
| Name | Quantity |
| Microtiter-plate coated with chloramphenicol (96 wells) | 1 pc |
| Chloramphenicol standards solutions with concentration 0 µg/l, 0,015 µg/l, 0,030 µg/l, 0,150 µg/l, 0,500 µg/l, 1,500 µg/l. | 6 pc × 1,8 mL |
| Conjugate | 8,0 mL |
| 10x Sample diluent buffer (concentrated)* | 25 mL |
| 20x Wash buffer (concentrated)* | 28 mL |
| Stop reagent* | 20 mL |
| TMB Substrate* | 12 mL |
| Extraction buffer solution A | 20 mL |
| Extraction buffer solution B | 20 mL |
| Sample balance buffer (concentrated) | 6 mL |
| Extraction buffer (concentrated salts mixture) | 30 g |
| Spike solution 100 ng/mL | 1 pc |
Components marked with * are interchangeable within their shelf- life if series number coincides.
Measuring detection limits
| Matrix | Detection limit | Range |
| Milk (raw, pasteurized, sterilized), reconstructed milk powdered | 0,030 µg/kg | 0,030 – 1,875 µg/kg |
| Cottage cheese, yogurt, kefir, sour cream, millk whey, reconstituted milk powdered whey, cheese, eggs | 0, 030 µg/kg | 0,030 – 1,5 µg/kg |
| Butter | 0,120 µg/kg | 0,012 – 6 µg/kg |
| Honey | 0,030 µg/kg | 0,030 – 1,5 µg/kg |
| Meat, ready-made meat products, by-products, fish, fish and shrimp products, salo, including speck, animal fats, canned meat and meat-growing products | 0,015 µg/kg | 0,015 – 0,75 µg/kg |
| Milk-based cocktails, ice cream, condensed milk | 0, 200 µg/kg | 0,200 – 18,8 µg/kg |
| Reconstituted powdered milk mixture for infant feeding | 0,038 µg/kg | 38 – 1,88 µg/kg |