Reagent kits for microRNA RT-PCR analysis

RT-PCR is the primary method of microRNA analysis in laboratory practice due to its reliability and cost-effectiveness. The kit implements the original technology of two-way priming of reverse transcription and PCR with two micro-RNA-specific primers (Androvic et al., 2017), which provides highest analysis accuracy. Optimization of the RT-primer structure and detection of amplification using a probe ensures reliable results and higher sensitivity of the assay compared to other technologies (Korobkina et al., 2018). This allows working with low concentration RNA samples